• Product name
  • Description
    Rabbit polyclonal to RYK
  • Host species
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 576-592 of Human RYK.

  • Positive control
    • Jurkat whole cell lysate (ab7899).


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab5518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 67.5 kDa (predicted molecular weight: 72 kDa).


  • Function
    May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.
  • Tissue specificity
    Observed in all the tissues examined.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 1 protein kinase domain.
    Contains 1 WIF domain.
  • Domain
    The extracellular WIF domain is responsible for Wnt binding.
  • Post-translational
    Proteolytically cleaved, in part by presenilin, in response to WNT3 stimulation. Cleavage occurs during neuronal differentiation.
  • Cellular localization
    Membrane. Nucleus. Cytoplasm. In cells that have undergone neuronal differentiation, the C-terminal cleaved part is translocated from the cytoplasm to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • D3S3195 antibody
    • ERK 3 antibody
    • Hydroxyaryl protein kinase antibody
    • JTK 5 antibody
    • JTK 5A antibody
    • JTK5 antibody
    • JTK5A antibody
    • JTK5A protein tyrosine kinase antibody
    • receptor like tyrosine kinase antibody
    • receptor like tyrosine kinase precursor antibody
    • RYK 1 antibody
    • Ryk antibody
    • RYK_HUMAN antibody
    • RYK1 antibody
    • Tyrosine protein kinase RYK antibody
    • Tyrosine protein kinase RYK precursor antibody
    • Tyrosine-protein kinase RYK antibody
    • Vik antibody
    see all


  • ab5518 at a 1/100 dilution staining approximately 67.5kDa band of RYK in Jurkat cell lysate by Western blot (ECL). ab5518 at a 1/100 dilution staining approximately 67.5kDa band of RYK in Jurkat cell lysate by Western blot (ECL).
  • ab5518 staining RYK in human hepatocarcinoma (HC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).


This product has been referenced in:
  • Skaria T  et al. Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells. Cell Adh Migr 11:24-38 (2017). Read more (PubMed: 27159116) »
See 1 Publication for this product

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