Other (Dot blot) Abreview for RNA polymerase II CTD repeat YSPTSPS peptide - phospho S2

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Review text: This peptide was used for dot blots to quality control anti-CTD antibodies. It was detected by ab5095 but not with anti-S5-CTD antibody.
Sample: Human Recombinant protein

Primary antibody (in addition to 'RNA polymerase II CTD repeat YSPTSPS peptide - phospho S2')
Primary antibody: Abcam primary antibody: Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody - ChIP Grade (ab5095)
Dilution: 1/3000

Secondary antibody
Name: Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/20000

Additional data
Notes: 0.1ug of peptide was spotted onto PVDF and membrane blocked with 2% BSA prior to antibody incubation.

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Submitted May 25 2012

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