Other (In vitro pull-down assay) Abreview for RNA polymerase II CTD repeat YSPTSPS peptide - phospho S2


Review text: This peptide was used in an in vitro pulldown assay. It was biotinylated and linked to streptavidin coated magnetic beads. This complex was then mixed with our proteins of interest under a variety of conditions. Proteins which bound to the CTD-S2p peptide were eluted and assessed by western blot.

This peptide was used along side ab18488, ab12795, and an unrelated non-abcam peptide and binding to each CTD was compared.
Sample: Human Recombinant protein

Primary antibody (in addition to 'RNA polymerase II CTD repeat YSPTSPS peptide - phospho S2')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used

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Submitted Aug 07 2012

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