Other (In vitro binding assay) Abreview for RNA polymerase II CTD repeat YSPTSPS peptide - phospho S2


Review text: This peptide was mixed with GST tagged recombinant proteins in vitro in binding buffer for 1 hour at room temp. Glutathione coated magnetic beads were then mixed into the buffer and removed after another hour incubation. The GST tagged recombinant proteins and any bound peptide was eluted by incubating with free glutathione. Eluted proteins were analyzed by western blot and peptides analyzed by dotblot.

This experiment was conducted with ab12795, ab18488 and an unrelated non-Abcam peptide as a negative control.
Sample: Human Recombinant protein

Primary antibody (in addition to 'RNA polymerase II CTD repeat YSPTSPS peptide - phospho S2')
Primary antibody: Abcam primary antibody: Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody - ChIP Grade (ab5095)
Dilution: 1/3000

Secondary antibody
Name: Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data
Notes: Ab5095 was used to detect peptides in this experiment.

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Submitted Aug 13 2012

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