Product name(S)-Ketoprofen, COX1/2 inhibitor
DescriptionHighly potent COX1/2 inhibitor. Non-steroidal anti-inflammatory drug (NSAID).
Biological descriptionHighly potent COX1/2 inhibitor (IC50 values are 19 and 54 nM for COX1 and 2 respectively). Non-steroidal anti-inflammatory drug (NSAID). Blood-brain barrier permeable.
Chemical name(S)-(+)-3-Benzoyl-α-methylbenzeneacetic acid
Storage instructionsStore at +4°C. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 100 mM and in ethanol to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
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- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Our Abpromise guarantee covers the use of ab141035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
DU145 cells were incubated at 37°C for 40h with vehicle control (0 µM) and different concentrations of (S)-Ketoprofen (ab141035) in DMSO. Decreased expression of pro-caspase 8 (ab49853) correlates with an increase in (S)-Ketoprofen concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab49853 at 1 μg/ml and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
This product has been referenced in:
- Ouellet M et al. Detergents profoundly affect inhibitor potencies against both cyclo-oxygenase isoforms. Biochem J 377:675-84 (2004). Read more (PubMed: 14510637) »
- Netter P et al. Diffusion of intramuscular ketoprofen into the cerebrospinal fluid. Eur J Clin Pharmacol 29:319-21 (1985). Read more (PubMed: 4076328) »
- Kantor TG Ketoprofen: a review of its pharmacologic and clinical properties. Pharmacotherapy 6:93-103 (0). Read more (PubMed: 3526298) »