- References (9)
Product name(S)-MCPG, Group I / II mGlu antagonist
DescriptionGroup I / II mGlu antagonist
Active isomer of (R,S)-MCPG (ab120033). Group I / II mGlu receptor antagonist with some activity reported at mGlu8.
Storage instructionsStore at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in 1.1eq. NaOH to 100mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab120059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab17722 staining FMRP in SK-N-SH cells treated with (S)-MCPG (ab120059), by ICC/IF. Decrease of FMRP expression correlates with increased concentration of (S)-MCPG, as described in literature.
The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120059 ((S)-MCPG) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab17722 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
This product has been referenced in:
- Schoepp DD et al. Pharmacological agents acting at subtypes of metabotropic glutamate receptors. Neuropharmacology 38:1431-76 (1999). Read more (PubMed: 10530808) »
- Sekiyama N et al. Structure-activity relationships of new agonists and antagonists of different metabotropic glutamate receptor subtypes. Br J Pharmacol 117:1493-503 (1996). Read more (PubMed: 8730745) »
- Bedingfield JS et al. Structure-activity relationships for a series of phenylglycine derivatives acting at metabotropic glutamate receptors (mGluRs). Br J Pharmacol 116:3323-9 (1995). Read more (PubMed: 8719814) »
- Thomsen C et al. Actions of phenylglycine analogs at subtypes of the metabotropic glutamate receptor family. Eur J Pharmacol 267:77-84 (1994). Read more (PubMed: 7515823) »