Product name(S)-MCPG, Group I / II mGlu antagonist
DescriptionGroup I / II mGlu antagonist
Active isomer of (R,S)-MCPG (ab120033). Group I / II mGlu receptor antagonist with some activity reported at mGlu8.
Storage instructionsStore at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in 1.1eq. NaOH to 100mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab120059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab17722 staining FMRP in SK-N-SH cells treated with (S)-MCPG (ab120059), by ICC/IF. Decrease of FMRP expression correlates with increased concentration of (S)-MCPG, as described in literature.
The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120059 ((S)-MCPG) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab17722 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab120059 has been referenced in 10 publications.
- Brandalise F & Gerber U Mossy fiber-evoked subthreshold responses induce timing-dependent plasticity at hippocampal CA3 recurrent synapses. Proc Natl Acad Sci U S A 111:4303-8 (2014). PubMed: 24550458
- Ledonne A et al. A continuous high frequency stimulation of the subthalamic nucleus determines a suppression of excitatory synaptic transmission in nigral dopaminergic neurons recorded in vitro. Exp Neurol 233:292-302 (2012). PubMed: 22056941
- Platt NJ et al. Striatal dopamine transmission is subtly modified in human A53Ta-synuclein overexpressing mice. PLoS One 7:e36397 (2012). PubMed: 22570709
- Threlfell S et al. Striatal dopamine release is triggered by synchronized activity in cholinergic interneurons. Neuron 75:58-64 (2012). PubMed: 22794260
- Gan J et al. Indirect modulation of neuronal excitability and synaptic transmission in the hippocampus by activation of proteinase-activated receptor-2. Br J Pharmacol 163:984-94 (2011). PubMed: 21366553
- Hartung H et al. Nitric oxide donors enhance the frequency dependence of dopamine release in nucleus accumbens. Neuropsychopharmacology 36:1811-22 (2011). PubMed: 21508928
- Fan W et al. Activation conditions for the induction of metabotropic glutamate receptor-dependent long-term depression in hippocampal CA1 pyramidal cells. J Neurosci 30:1471-5 (2010). PubMed: 20107074
- Threlfell S et al. Striatal muscarinic receptors promote activity dependence of dopamine transmission via distinct receptor subtypes on cholinergic interneurons in ventral versus dorsal striatum. J Neurosci 30:3398-408 (2010). PubMed: 20203199
- Andrade AL & Rossi DJ Simulated ischaemia induces Ca2+-independent glutamatergic vesicle release through actin filament depolymerization in area CA1 of the hippocampus. J Physiol 588:1499-514 (2010). PubMed: 20211977
- Robberechts Q et al. Long-term depression at parallel fiber to Golgi cell synapses. J Neurophysiol 104:3413-23 (2010). PubMed: 20861429