Overview

  • Product name

    Anti-S-nitrosocysteine antibody [HY8E12]
  • Description

    Mouse monoclonal [HY8E12] to S-nitrosocysteine
  • Host species

    Mouse
  • Specificity

    ab94930 targets conjugated S-nitrosocysteine, it does not recognize free S-nitrosocysteine.
  • Tested applications

    Suitable for: ICC, WB, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to S-nitrosocysteine conjugated to Bovine Serum Albumin (BSA).

Properties

Applications

Our Abpromise guarantee covers the use of ab94930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/1000 - 1/5000.

ab131369 can be used as blocking peptide.

WB 1/1000 - 1/2000.
IHC-P 1/1000 - 1/5000.

Fixation of tissue for use with this antibody should be done with glutaraldehyde. The use of paraformaldehyde in conjunction with glutaraldehyde may improve staining results.

ab131369 can be used as blocking peptide.

IHC-Fr Use at an assay dependent concentration.

ab131369 can be used as blocking peptide.

Target

  • Relevance

    All mammalian cells contain low levels of nitrosylated proteins, that are thought to be regulated by Snitrosylation and denitrosylation. S nitrosylation of proteins serves as a ubiquitous post-translational modification that dynamically regulates a broad functional spectrum of proteins. The majority of these proteins are regulated by S nitrosylation on a single critical cysteine residue within an acidic/basic or hydrophobic structural motif, that may also be subject to oxygen or glutathione dependent modification, suggesting that S nitrosylation is a prototypic redox signal.

Images

  • Mouse pancreatic islets were treated with 30 nm GLP-1 for 10 min prior to lysis. GCK was immunoprecipitated and assessed for S-nitrosocysteine by Western blotting using ab94930.

References

This product has been referenced in:

  • Waller KA  et al. Friction-Induced Mitochondrial Dysregulation Contributes to Joint Deterioration in Prg4 Knockout Mice. Int J Mol Sci 18:N/A (2017). WB . Read more (PubMed: 28604608) »
  • Gupta A  et al. PARK2 Depletion Connects Energy and Oxidative Stress to PI3K/Akt Activation via PTEN S-Nitrosylation. Mol Cell 65:999-1013.e7 (2017). Read more (PubMed: 28306514) »
See all 8 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Melanoma cancer)
Loading amount
30 µg
Specification
Melanoma cancer
Treatment
5gy Irradiation
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 25°C

Ms. Seontae Kim

Verified customer

Submitted Feb 19 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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