Overview

  • Product name
    Anti-S-nitrosocysteine antibody [HY8E12]
  • Description
    Mouse monoclonal [HY8E12] to S-nitrosocysteine
  • Host species
    Mouse
  • Specificity
    ab94930 targets conjugated S-nitrosocysteine, it does not recognize free S-nitrosocysteine.
  • Tested applications
    Suitable for: ICC, WB, IHC-P, IHC-Frmore details
  • Immunogen

    Chemical/ Small Molecule corresponding to S-nitrosocysteine conjugated to Bovine Serum Albumin (BSA).

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

Applications

Our Abpromise guarantee covers the use of ab94930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/1000 - 1/5000.

ab131369 can be used as blocking peptide.

WB 1/1000 - 1/2000.
IHC-P 1/1000 - 1/5000.

Fixation of tissue for use with this antibody should be done with glutaraldehyde. The use of paraformaldehyde in conjunction with glutaraldehyde may improve staining results.

ab131369 can be used as blocking peptide.

IHC-Fr Use at an assay dependent concentration.

ab131369 can be used as blocking peptide.

Target

  • Relevance
    All mammalian cells contain low levels of nitrosylated proteins, that are thought to be regulated by Snitrosylation and denitrosylation. S nitrosylation of proteins serves as a ubiquitous post-translational modification that dynamically regulates a broad functional spectrum of proteins. The majority of these proteins are regulated by S nitrosylation on a single critical cysteine residue within an acidic/basic or hydrophobic structural motif, that may also be subject to oxygen or glutathione dependent modification, suggesting that S nitrosylation is a prototypic redox signal.

Images

  • Mouse pancreatic islets were treated with 30 nm GLP-1 for 10 min prior to lysis. GCK was immunoprecipitated and assessed for S-nitrosocysteine by Western blotting using ab94930.

References

This product has been referenced in:
  • Waller KA  et al. Friction-Induced Mitochondrial Dysregulation Contributes to Joint Deterioration in Prg4 Knockout Mice. Int J Mol Sci 18:N/A (2017). WB . Read more (PubMed: 28604608) »
  • Bahnson ES  et al. Targeted Nitric Oxide Delivery by Supramolecular Nanofibers for the Prevention of Restenosis After Arterial Injury. Antioxid Redox Signal 24:401-18 (2016). Read more (PubMed: 26593400) »

See all 4 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Melanoma cancer)
Loading amount
30 µg
Specification
Melanoma cancer
Treatment
5gy Irradiation
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Ms. Seontae Kim

Verified customer

Submitted Feb 19 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up