S. pombe Histone H2A (phospho S129) peptide (ab17576)

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Western blot - <em>S. pombe</em> Histone H2A (phospho S129) peptide (ab17576)

    Key features and details

    • Suitable for: Blocking

    Description

    Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab17576 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Blocking - Blocking peptide for Anti-Histone H2A (phospho S129) antibody (ab17353)

    • Form

      Liquid

    • Additional notes

      - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
      - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
      - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
      - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
      - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      Information available upon request.

    General Info

    • Alternative names

      • H2A
      • H2a 615
      • H2A GL101
      • H2A histone family member A
      • H2A.1
      • H2A.2
      • H2A/a
      • H2A/m
      • H2A/O
      • H2A/q
      • H2A1B_HUMAN
      • H2AFA
      • H2AFE
      • H2AFL
      • H2AFM
      • H2AFO
      • H2AFQ
      • HIST1H2AE
      • HIST1H2AJ
      • HIST2H2AA
      • HIST2H2AA3
      • HIST2H2AB
      • HIST2H2AC
      • Histone 1 H2ae
      • Histone 2 H2aa3
      • Histone 2 H2ab
      • Histone 2 H2ac
      • Histone H2A type 1 B
      • Histone H2A type 1 C
      • Histone H2A type 1 E
      • Histone H2A type 1 J
      • Histone H2A type 1-B/E
      • Histone H2A.2
      • Histone H2A/a
      • Histone H2A/m
      • MGC74460
      see all

    • Function

      Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

    • Sequence similarities

      Belongs to the histone H2A family.

    • Post-translational
      modifications

      The chromatin-associated form is phosphorylated on Thr-121 during mitosis.
      Deiminated on Arg-4 in granulocytes upon calcium entry.
      Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
      Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
      Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.

    • Cellular localization

      Nucleus. Chromosome.
    • Information by UniProt

    Images

    • Western blot - <em>S. pombe</em> Histone H2A (phospho S129) peptide (ab17576)
      Western blot - S. pombe Histone H2A (phospho S129) peptide (ab17576)
      ab17353 recognised a band of the predicted size in S. pombe lysate (lane 1). This band was blocked by the immunising peptide (lane 2) but not by the unmodified version of the immunising peptide (lane 3). This suggests specificity for the modified residue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab17576? Please let us know so that we can cite the reference in this datasheet.

    ab17576 has been referenced in 1 publication.

    • Fletcher J  et al. Nutrient Limitation Inactivates Mrc1-to-Cds1 Checkpoint Signalling in Schizosaccharomyces pombe. Cells 7:N/A (2018). PubMed: 29473861

    Customer reviews and Q&As

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    Other (Dot Blot) Abreview for Histone H2A (yeast) peptide - phospho S129

     Excellent
    Abreviews
    Application
    Other - Do not use

    Review text: I used this product to do a dot blot specificity test for cross reactivity of modified histone antibodies and everything worked great. I made two dilutions of the peptide and spotted it on a nitrocellulose membrane using a Bio-Dot apparatus. Once the peptides were bound to the membrane I incubated with the appropriate primary and secondary antibodies at an assay dependent concentration and developed using ECL.

    No primary antibody directed towards H2AS129phos was used in this dot blot assay. This peptide was chosen to ensure there was no cross reactivity between the antibodies we are using on a regular basis. We have yet to see any cross reactivity with this peptide using 12+ antibodies directed towards histone H3 modified antibodies (some from Abcam some not).
    Sample: Human Purified protein

    Primary antibody (in addition to 'Histone H2A (yeast) peptide - phospho S129')
    Primary antibody: None used

    Secondary antibody
    Secondary antibody: None used

    Abcam user community

    Verified customer

    Submitted Nov 01 2010

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