Overview

  • Product name

  • Description

    Rabbit polyclonal to S tag
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    This information is considered to be commercially sensitive.

  • Positive control

    • S tag transfected 293T cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000.
IP Use at an assay dependent concentration.

Use 2.5µg.

Target

  • Relevance

    S tag is an epitope tag composed of a 15 residue peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A.
  • Alternative names

    • KETAAAKFERQHMDS tag antibody antibody
    • S peptide antibody
    • S peptide epitope tag antibody

Images

  • All lanes : Anti-S tag antibody (ab183674) at 1/5000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : S tag transfected 293T whole cell lysate

    Lysates/proteins at 30 µg per lane.


    12% SDS-PAGE

  • S tag antibody ab183674 immunoprecipitates S tag protein in IP experiments. IP Sample: 650 µg whole cell lysate of S tag protein expressing HeLa cells (1), 20 μg whole cell lysate of transfected HeLa cells (2), Control with 2.5 μg of preimmune rabbit IgG (3). Immunoprecipitation of S tag protein by 2.5 μg of ab183674. 12% SDS-PAGE. The immunoprecipitated S tag protein was detected by ab183674 diluted at 1/10000. Anti-rabbit IgG was used as a secondary reagent.

References

ab183674 has not yet been referenced specifically in any publications.

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