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  1. Link

    s-tag-antibody-ab19321.pdf

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Tags & Cell Markers Epitope Tags Other Tags S Tag
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Anti-S tag antibody (ab19321)

  • Datasheet
  • SDS
Submit a review Submit a question References (11)

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Western blot - Anti-S tag antibody (ab19321)

    Key features and details

    • Goat polyclonal to S tag
    • Suitable for: ELISA, WB, ICC, ICC/IF
    • Reacts with: Species independent
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-S tag antibody [EPR12996] (ab180958)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-S tag antibody
      See all S tag primary antibodies
    • Description

      Goat polyclonal to S tag
    • Host species

      Goat
    • Tested applications

      Suitable for: ELISA, WB, ICC, ICC/IFmore details
    • Species reactivity

      Reacts with: Species independent
    • Immunogen

      Synthetic peptide:

      KETAAAKFERQHMDS

      conjugated to KLH.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 6.8
      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Tags & Cell Markers
      • Epitope Tags
      • Other Tags
      • S Tag

    Associated products

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab19321 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA
    1/100 - 1/500. Primary: 1/1000 - 1/30000.
    WB
    1/1000 - 1/30000. Predicted molecular weight: 23 kDa.
    ICC
    1/100 - 1/400.
    ICC/IF
    Use at an assay dependent concentration. PubMed: 21518912
    Notes
    ELISA
    1/100 - 1/500. Primary: 1/1000 - 1/30000.
    WB
    1/1000 - 1/30000. Predicted molecular weight: 23 kDa.
    ICC
    1/100 - 1/400.
    ICC/IF
    Use at an assay dependent concentration. PubMed: 21518912

    Target

    • Relevance

      S tag is an epitope tag composed of a 15 residue peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A.
    • Alternative names

      • KETAAAKFERQHMDS tag antibody antibody
      • S peptide antibody
      • S peptide epitope tag antibody

    Images

    • Western blot - Anti-S tag antibody (ab19321)
      Western blot - Anti-S tag antibody (ab19321)
      All lanes : Anti-S tag antibody (ab19321) at 0.04 µg/ml

      Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
      Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
      Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg

      Predicted band size: 23 kDa


      Exposure time: 30 seconds

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (11)

    Publishing research using ab19321? Please let us know so that we can cite the reference in this datasheet.

    ab19321 has been referenced in 11 publications.

    • Lin DL  et al. The ER Membrane Protein Complex Promotes Biogenesis of Dengue and Zika Virus Non-structural Multi-pass Transmembrane Proteins to Support Infection. Cell Rep 27:1666-1674.e4 (2019). PubMed: 31067454
    • Shen K  et al. Dual Role of Ribosome-Binding Domain of NAC as a Potent Suppressor of Protein Aggregation and Aging-Related Proteinopathies. Mol Cell 74:729-741.e7 (2019). PubMed: 30982745
    • David M  et al. Identification and characterization of highly versatile peptide-vectors that bind non-competitively to the low-density lipoprotein receptor for in vivo targeting and delivery of small molecules and protein cargos. PLoS One 13:e0191052 (2018). ICC/IF, ELISA . PubMed: 29485998
    • Gallmetzer A  et al. Reversible Oxidation of a Conserved Methionine in the Nuclear Export Sequence Determines Subcellular Distribution and Activity of the Fungal Nitrate Regulator NirA. PLoS Genet 11:e1005297 (2015). WB . PubMed: 26132230
    • Adeyo O  et al. Palmoplantar Keratoderma along with Neuromuscular and Metabolic Phenotypes in Slurp1-Deficient Mice. J Invest Dermatol N/A:N/A (2014). PubMed: 24499735
    View all Publications for this product

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab19321.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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