Anti-S tag antibody (ab19321)
Key features and details
- Goat polyclonal to S tag
- Suitable for: ELISA, WB, ICC, ICC/IF
- Reacts with: Species independent
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-S tag antibody
See all S tag primary antibodies -
Description
Goat polyclonal to S tag -
Host species
Goat -
Tested applications
Suitable for: ELISA, WB, ICC, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab19321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
1/100 - 1/500. Primary: 1/1000 - 1/30000.
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| WB |
1/1000 - 1/30000. Predicted molecular weight: 23 kDa.
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| ICC |
1/100 - 1/400.
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| ICC/IF |
Use at an assay dependent concentration. PubMed: 21518912
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| Notes |
|---|
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ELISA
1/100 - 1/500. Primary: 1/1000 - 1/30000. |
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WB
1/1000 - 1/30000. Predicted molecular weight: 23 kDa. |
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ICC
1/100 - 1/400. |
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ICC/IF
Use at an assay dependent concentration. PubMed: 21518912 |
Target
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Relevance
S tag is an epitope tag composed of a 15 residue peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A. -
Alternative names
- KETAAAKFERQHMDS tag antibody antibody
- S peptide antibody
- S peptide epitope tag antibody
Images
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Western blot - Anti-S tag antibody (ab19321)All lanes : Anti-S tag antibody (ab19321) at 0.04 µg/ml
Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg
Predicted band size: 23 kDa
Exposure time: 30 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab19321 has been referenced in 11 publications.
- Lin DL et al. The ER Membrane Protein Complex Promotes Biogenesis of Dengue and Zika Virus Non-structural Multi-pass Transmembrane Proteins to Support Infection. Cell Rep 27:1666-1674.e4 (2019). PubMed: 31067454
- Shen K et al. Dual Role of Ribosome-Binding Domain of NAC as a Potent Suppressor of Protein Aggregation and Aging-Related Proteinopathies. Mol Cell 74:729-741.e7 (2019). PubMed: 30982745
- David M et al. Identification and characterization of highly versatile peptide-vectors that bind non-competitively to the low-density lipoprotein receptor for in vivo targeting and delivery of small molecules and protein cargos. PLoS One 13:e0191052 (2018). ICC/IF, ELISA . PubMed: 29485998
- Gallmetzer A et al. Reversible Oxidation of a Conserved Methionine in the Nuclear Export Sequence Determines Subcellular Distribution and Activity of the Fungal Nitrate Regulator NirA. PLoS Genet 11:e1005297 (2015). WB . PubMed: 26132230
- Adeyo O et al. Palmoplantar Keratoderma along with Neuromuscular and Metabolic Phenotypes in Slurp1-Deficient Mice. J Invest Dermatol N/A:N/A (2014). PubMed: 24499735