Overview

  • Product name

    Anti-S100 alpha 2/S100A2 antibody
    See all S100 alpha 2/S100A2 primary antibodies
  • Description

    Rabbit polyclonal to S100 alpha 2/S100A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Full length protein corresponding to Human S100 alpha 2/S100A2 aa 1-98. (AAH02829)

  • Positive control

    • Human pancreas and Mouse liver tissue lysates; S100 alpha 2/S100A2 transfected 293T cell lysate
  • General notes

     This product was previously labelled as S100 alpha 2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab103423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 11 kDa.

Target

  • Function

    May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes. May also play a role in suppressing tumor cell growth.
  • Tissue specificity

    A subset of epithelial cells including normal human mammary epithelial cells and keratinocytes.
  • Sequence similarities

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Developmental stage

    Preferentially expressed in normal human mammary epithelial cells as opposed to tumor-derived ones. The level of S100L was shown to correlate inversely with tumor progression.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAN19 antibody
    • MGC111539 antibody
    • Protein S 100L antibody
    • Protein S-100L antibody
    • Protein S100 A2 antibody
    • Protein S100-A2 antibody
    • Protein S100L antibody
    • S100 A2 antibody
    • S100 calcium binding protein A2 antibody
    • S100 calcium-binding protein A2 antibody
    • S100A2 antibody
    • S100L antibody
    • S10A2_HUMAN antibody
    see all

Images

  • Anti-S100 alpha 2/S100A2 antibody (ab103423) at 1/500 dilution + Human pancres tissue lysate at 50 µg

    Predicted band size: 11 kDa

  • Anti-S100 alpha 2/S100A2 antibody (ab103423) at 1/500 dilution + Mouse liver tissue lysate at 50 µg

    Predicted band size: 11 kDa

  • All lanes : Anti-S100 alpha 2/S100A2 antibody (ab103423) at 1/500 dilution

    Lane 1 : S100 alpha 2/S100A2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 11 kDa

References

ab103423 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (primary hippocampal neurons)
Specification
primary hippocampal neurons
Fixative
Paraformaldehyde
Permeabilization
Yes - ice-cold methanol
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 15 2012

Application
Western blot
Sample
Mouse Cell lysate - whole cell (primary hippocampal neurons)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Specification
primary hippocampal neurons
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 14 2012

Answer

Ref: Problem with a product. ChIP inquiry

Thank you for your enquiry and your interest in our products.

I can confirm that as the on-line datasheet suggeststhis antibody has only been tested for Western blot application. It has not been used in ChIP application.

If you need any further assistance in the future, please do not hesitate to contact me.

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Answer

Thank you for your email.
DISCOUNT CODE: xxx for ab103423
DISCOUNT CODE: xxx for ab93853
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab103423 and ab93853 in ICC/IF. Each code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit each an Abreview for ICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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