Key features and details
- Rabbit polyclonal to S100 alpha 2/S100A2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-S100 alpha 2/S100A2 antibody
See all S100 alpha 2/S100A2 primary antibodies
DescriptionRabbit polyclonal to S100 alpha 2/S100A2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
- WB: RT4 cell lysate. IHC-P: Human esophagus, kidney, uterine cervix and skin tissue.
This product was previously labelled as S100 alpha 2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab244524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionMay function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes. May also play a role in suppressing tumor cell growth.
Tissue specificityA subset of epithelial cells including normal human mammary epithelial cells and keratinocytes.
Sequence similaritiesBelongs to the S-100 family.
Contains 2 EF-hand domains.
Developmental stagePreferentially expressed in normal human mammary epithelial cells as opposed to tumor-derived ones. The level of S100L was shown to correlate inversely with tumor progression.
- Information by UniProt
- CAN19 antibody
- MGC111539 antibody
- Protein S 100L antibody
Anti-S100 alpha 2/S100A2 antibody (ab244524) at 0.4 µg/ml + RT4 (Human urinary bladder cancer cell line) cell lysate
Formalin-fixed, paraffin-embedded human skin tissue stained for 2/S100A2 with ab244524 at a 1/500 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human kidney tissue stained for 2/S100A2 with ab244524 at a 1/500 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human uterine cervix tissue stained for 2/S100A2 with ab244524 at a 1/500 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human esophagus tissue stained for 2/S100A2 with ab244524 at a 1/500 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human liver tissue shows absence of immunoreactivity for 2/S100A2 when incubated with ab244524 at a 1/500 dilution (negative control).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244524 has not yet been referenced specifically in any publications.