The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.
Belongs to the S-100 family. Contains 2 EF-hand domains.