Anti-S100 alpha 6/PRA antibody (ab154955)
Key features and details
- Rabbit polyclonal to S100 alpha 6/PRA
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-S100 alpha 6/PRA antibody
See all S100 alpha 6/PRA primary antibodies -
Description
Rabbit polyclonal to S100 alpha 6/PRA -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human S100 alpha 6/PRA aa 1-90.
Database link: P06703 -
Positive control
- U-87 MG whole cell lysate; A431 cells.
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General notes
This product was previously labelled as S100 alpha 6
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab154955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 10 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative. -
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Nucleus envelope. Cytoplasm. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 6277 Human
- Omim: 114110 Human
- SwissProt: P06703 Human
- Unigene: 275243 Human
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Alternative names
- 2A9 antibody
- 5B10 antibody
- CABP antibody
see all
Images
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Western blot - Anti-S100 alpha 6/PRA antibody (ab154955)Anti-S100 alpha 6/PRA antibody (ab154955) at 1/5000 dilution + U-87 MG whole cell lysate at 30 µg
Predicted band size: 10 kDa
15% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody (ab154955)Immunofluorescence analysis of paraformaldehyde-fixed A431 cells labeling S100 alpha 6/PRA with ab154955 at 1/500 dilution (green). Lower image shows cells co-stained with Hoechst 33342.
Protocols
References (0)
ab154955 has not yet been referenced specifically in any publications.
