Recombinant
RabMAb

Recombinant Anti-S100 alpha 6/PRA antibody [EPNCIR121] (ab134149)

Overview

  • Product name

    Anti-S100 alpha 6/PRA antibody [EPNCIR121]
    See all S100 alpha 6/PRA primary antibodies
  • Description

    Rabbit monoclonal [EPNCIR121] to S100 alpha 6/PRA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human S100 alpha 6/PRA.
    Database link: P06703

  • Positive control

    • Fetal lung, A549, ovary cancer, SH-SY5Y, and Human T-78 cell lysates, HeLa cells
  • General notes

     

     

     This product was previously labelled as S100 alpha 6

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 10 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.
    • Sequence similarities

      Belongs to the S-100 family.
      Contains 2 EF-hand domains.
    • Post-translational
      modifications

      The N-terminus is blocked.
    • Cellular localization

      Nucleus envelope. Cytoplasm. Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 2A9 antibody
      • 5B10 antibody
      • CABP antibody
      • CACY antibody
      • Calcyclin antibody
      • Growth factor inducible protein 2A9 antibody
      • Growth factor-inducible protein 2A9 antibody
      • MLN 4 antibody
      • MLN4 antibody
      • OTTHUMP00000015472 antibody
      • OTTHUMP00000015473 antibody
      • PRA antibody
      • PRAGrowth factor inducible protein 2A9 antibody
      • Prolactin receptor associated protein antibody
      • Prolactin receptor-associated protein antibody
      • Protein S100 A6 antibody
      • Protein S100-A6 antibody
      • S100 A6 antibody
      • S100 calcium binding protein A6 (calcyclin) antibody
      • S100 calcium binding protein A6 antibody
      • S100 calcium-binding protein A6 antibody
      • S100A6 antibody
      • S10A6_HUMAN antibody
      see all

    Images

    • All lanes : Anti-S100 alpha 6/PRA antibody [EPNCIR121] (ab134149) at 1/1000 dilution

      Lane 1 : Fetal lung cell lysates
      Lane 2 : A549 cell lysates
      Lane 3 : Ovary cancer cell lysates
      Lane 4 : SH-SY5Y cell lysates
      Lane 5 : Human T-78 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 10 kDa

    • Overlay histogram showing SHSY-5Y cells stained with ab134149 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134149, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Immunofluorescence analysis of HeLa cells labelled with ab134149 at 1/100 dilution.

    References

    This product has been referenced in:

    • Beyaz S  et al. The histone demethylase UTX regulates the lineage-specific epigenetic program of invariant natural killer T cells. Nat Immunol 18:184-195 (2017). Read more (PubMed: 27992400) »
    • Shimamoto S  et al. Ca2+/S100 proteins act as upstream regulators of the chaperone-associated ubiquitin ligase CHIP (C terminus of Hsc70-interacting protein). J Biol Chem 288:7158-68 (2013). WB . Read more (PubMed: 23344957) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134149.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up