Product nameAnti-S100 alpha 6/PRA antibody [EPNCIR121]
See all S100 alpha 6/PRA primary antibodies
DescriptionRabbit monoclonal [EPNCIR121] to S100 alpha 6/PRA
Tested applicationsSuitable for: WB, IP, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
corresponding to Human S100 alpha 6/PRA.
Database link: P06703
- Fetal lung, A549, ovary cancer, SH-SY5Y, and Human T-78 cell lysates, HeLa cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 10 kDa.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
FunctionMay function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.
Sequence similaritiesBelongs to the S-100 family.
Contains 2 EF-hand domains.
modificationsThe N-terminus is blocked.
Cellular localizationNucleus envelope. Cytoplasm. Cell membrane.
- Information by UniProt
- 2A9 antibody
- 5B10 antibody
- CABP antibody
All lanes : Anti-S100 alpha 6/PRA antibody [EPNCIR121] (ab134149) at 1/1000 dilution
Lane 1 : Fetal lung cell lysates
Lane 2 : A549 cell lysates
Lane 3 : Ovary cancer cell lysates
Lane 4 : SH-SY5Y cell lysates
Lane 5 : Human T-78 cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 10 kDa
Overlay histogram showing SHSY-5Y cells stained with ab134149 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134149, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunofluorescence analysis of HeLa cells labelled with ab134149 at 1/100 dilution.
This product has been referenced in:
- Beyaz S et al. The histone demethylase UTX regulates the lineage-specific epigenetic program of invariant natural killer T cells. Nat Immunol 18:184-195 (2017). Read more (PubMed: 27992400) »
- Shimamoto S et al. Ca2+/S100 proteins act as upstream regulators of the chaperone-associated ubiquitin ligase CHIP (C terminus of Hsc70-interacting protein). J Biol Chem 288:7158-68 (2013). WB . Read more (PubMed: 23344957) »