Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13084-69] to S100 alpha 6/PRA
- Suitable for: WB, IP, Flow Cyt, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-S100 alpha 6/PRA antibody [EPR13084-69]
See all S100 alpha 6/PRA primary antibodies
DescriptionRabbit monoclonal [EPR13084-69] to S100 alpha 6/PRA
Tested applicationsSuitable for: WB, IP, Flow Cyt, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human S100 alpha 6/PRA aa 1 to the C-terminus.
Database link: P06703
- HeLa, SH-SY5Y, Human fetal lung and A549 lysates; Human colonic carcinoma tissue; A549 cells; HeLa cells.
This product was previously labelled as S100 alpha 6
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionMay function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.
Sequence similaritiesBelongs to the S-100 family.
Contains 2 EF-hand domains.
modificationsThe N-terminus is blocked.
Cellular localizationNucleus envelope. Cytoplasm. Cell membrane.
- Information by UniProt
- 2A9 antibody
- 5B10 antibody
- CABP antibody
All lanes : Anti-S100 alpha 6/PRA antibody [EPR13084-69] (ab181975) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : Human fetal lung lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 10 kDa
Observed band size: 10 kDa
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labelling S100 alpha 6/PRA with ab181975 at 1/2000 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in A549 cells. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling S100 alpha 6/PRA with ab181975 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling S100 alpha 6/PRA with ab181975 at 1/30 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (greeen), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Western blot analysis of HeLa cell lysate immunoprecipitated with ab181975 at 1/60 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody used at 1/1000 dilution.
ab181975 has been referenced in 2 publications.
- Kjell J et al. Defining the Adult Neural Stem Cell Niche Proteome Identifies Key Regulators of Adult Neurogenesis. Cell Stem Cell 26:277-293.e8 (2020). PubMed: 32032526
- Gocheva V et al. Quantitative proteomics identify Tenascin-C as a promoter of lung cancer progression and contributor to a signature prognostic of patient survival. Proc Natl Acad Sci U S A 114:E5625-E5634 (2017). PubMed: 28652369