Recombinant
RabMAb

Recombinant Anti-S100 alpha 6/PRA antibody [EPR13084] (ab181974)

Overview

  • Product name

    Anti-S100 alpha 6/PRA antibody [EPR13084]
    See all S100 alpha 6/PRA primary antibodies
  • Description

    Rabbit monoclonal [EPR13084] to S100 alpha 6/PRA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human S100 alpha 6/PRA aa 1 to the C-terminus.
    Database link: P06703

  • Positive control

    • HeLa and A549 cell lysates; Human fetal lung lysate; Human colonic carcinoma tissue; A549 and HeLa cells.
  • General notes

     

     

     This product was previously labelled as S100 alpha 6

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/2000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
ICC/IF 1/500.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.
  • Sequence similarities

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Nucleus envelope. Cytoplasm. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2A9 antibody
    • 5B10 antibody
    • CABP antibody
    • CACY antibody
    • Calcyclin antibody
    • Growth factor inducible protein 2A9 antibody
    • Growth factor-inducible protein 2A9 antibody
    • MLN 4 antibody
    • MLN4 antibody
    • OTTHUMP00000015472 antibody
    • OTTHUMP00000015473 antibody
    • PRA antibody
    • PRAGrowth factor inducible protein 2A9 antibody
    • Prolactin receptor associated protein antibody
    • Prolactin receptor-associated protein antibody
    • Protein S100 A6 antibody
    • Protein S100-A6 antibody
    • S100 A6 antibody
    • S100 calcium binding protein A6 (calcyclin) antibody
    • S100 calcium binding protein A6 antibody
    • S100 calcium-binding protein A6 antibody
    • S100A6 antibody
    • S10A6_HUMAN antibody
    see all

Images

  • All lanes : Anti-S100 alpha 6/PRA antibody [EPR13084] (ab181974) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 10 kDa
    Observed band size: 10 kDa

  • Anti-S100 alpha 6/PRA antibody [EPR13084] (ab181974) at 1/2000 dilution + Human fetal lung lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 10 kDa
    Observed band size: 10 kDa

  • Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling S100 alpha 6/PRA with ab181974 at 1/50 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling S100 alpha 6/PRA with ab181974 at 1/500 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/200 dilution (green). Dapi staining (blue).

  • Immunofluorescent analysis of acetone-fixed HeLa cells labeling S100 alpha 6/PRA with ab181974 at 1/500 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/200 dilution (red). Dapi staining (blue).

References

ab181974 has not yet been referenced specifically in any publications.

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