Recombinant Anti-S100 alpha 6/PRA antibody [EPR13084] (ab181974)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13084] to S100 alpha 6/PRA
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-S100 alpha 6/PRA antibody [EPR13084]
See all S100 alpha 6/PRA primary antibodies -
Description
Rabbit monoclonal [EPR13084] to S100 alpha 6/PRA -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human S100 alpha 6/PRA aa 1 to the C-terminus.
Database link: P06703 -
Positive control
- HeLa and A549 cell lysates; Human fetal lung lysate; Human colonic carcinoma tissue; A549 and HeLa cells.
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General notes
This product was previously labelled as S100 alpha 6
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13084 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab181974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | 1/25 - 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
WB | 1/1000 - 1/2000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa). | |
ICC/IF | 1/500. |
Target
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Function
May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative. -
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Nucleus envelope. Cytoplasm. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 6277 Human
- Omim: 114110 Human
- SwissProt: P06703 Human
- Unigene: 275243 Human
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Alternative names
- 2A9 antibody
- 5B10 antibody
- CABP antibody
see all
Images
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All lanes : Anti-S100 alpha 6/PRA antibody [EPR13084] (ab181974) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 10 kDa
Observed band size: 10 kDa -
Anti-S100 alpha 6/PRA antibody [EPR13084] (ab181974) at 1/2000 dilution + Human fetal lung lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 10 kDa
Observed band size: 10 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 6/PRA antibody [EPR13084] (ab181974)
Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling S100 alpha 6/PRA with ab181974 at 1/50 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling S100 alpha 6/PRA with ab181974 at 1/500 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/200 dilution (green). Dapi staining (blue).
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Immunofluorescent analysis of acetone-fixed HeLa cells labeling S100 alpha 6/PRA with ab181974 at 1/500 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/200 dilution (red). Dapi staining (blue).
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab181974 has not yet been referenced specifically in any publications.