Overview

  • Product name

    Anti-S100 alpha antibody [1942] - BSA and Azide free
    See all S100 alpha primary antibodies
  • Description

    Mouse monoclonal [1942] to S100 alpha - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Orangutan
  • Immunogen

    Recombinant full length protein corresponding to Human S100 alpha aa 1-94.
    Sequence:

    MGSELETAMETLINVFHAHSGKEGDKYKLSKKELKELLQTELSGFLDAQK DVDAVDKVMKELDENGDGEVDFQEYVVLVAALTVACNNFFWENS


    Database link: P23297

  • Positive control

    • WB: Human melanoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab212818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 2 µg/ml.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.

Target

  • Function

    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
  • Tissue specificity

    Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • Sequence similarities

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Bpb antibody
    • NEF antibody
    • Protein S100-A1 antibody
    • S-100 protein alpha chain antibody
    • S-100 protein subunit alpha antibody
    • S100 alpha antibody
    • S100 Alpha Chain antibody
    • S100 antibody
    • S100 Beta Chain antibody
    • S100 Calcium Binding Protein A1 antibody
    • S100 Calcium Binding Protein B antibody
    • S100 Calcium Binding Protein Beta Neural antibody
    • S100 calcium-binding protein A1 antibody
    • S100 protein alpha polypeptide antibody
    • S100A antibody
    • s100a1 antibody
    • S10A1_HUMAN antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human melanoma tissue stained for S100 alpha with ab212818 at 2 µg/ml in immunohistochemical analysis.

References

ab212818 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab212818.
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