Key features and details
- Rabbit polyclonal to S100 alpha
- Suitable for: ELISA, IP, WB, IHC-P, ICC, ICC/IF
- Reacts with: Mouse, Rat, Cow, Dog, Human, Pig
- Isotype: IgG
Product nameAnti-S100 alpha antibody
See all S100 alpha primary antibodies
DescriptionRabbit polyclonal to S100 alpha
Tested applicationsSuitable for: ELISA, IP, WB, IHC-P, ICC, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Cow, Dog, Human, Pig
Full length native protein (purified) corresponding to Human S100 alpha. Purified S100-alpha protein from human pectoral muscle cells.
- Human muscle.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 3% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab11428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IP||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 0.1 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa). Use at a concentration of 0.1 µg/ml. Detects a band of approximately 10 kDa, representing S100-alpha from human muscle. S100 protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane.|
|IHC-P||Use a concentration of 1 µg/ml.|
|ICC||1/50 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionWeakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
Tissue specificityHighly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
Sequence similaritiesBelongs to the S-100 family.
Contains 2 EF-hand domains.
- Information by UniProt
- Bpb antibody
- NEF antibody
- Protein S100-A1 antibody
ab11428 (2µg/ml) staining S100 alpha in human breast tissue using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear and cytoplasmic compartment within the breast ductal regions
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ICC/IF image of ab11428 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11428, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab11428 labelling S100 alpha (red) in HEK293T cells by Immunocytochemistry/Immunofluorescence. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were incubated with primary antibody (1:100 in blocking buffer) overnight at 4ºC. A fluorophore-conjugated goat anti-rabbit IgG (1:200) was used as the secondary antibody (1 hour at room temperature). Blue (DAPI) - nuclei. Images were taken at 40X magnification.
A Western blot of S100-alpha from human ovary extract (50µg) using ab11428 at 1/1000 dilution. A secondary goat anti rabbit HRP conjugate was used to visualise.
ab11428 has been referenced in 4 publications.
- Zhang T et al. Six1 is essential for differentiation and patterning of the mammalian auditory sensory epithelium. PLoS Genet 13:e1006967 (2017). PubMed: 28892484
- Conner JR et al. HNF1ß and S100A1 are useful biomarkers for distinguishing renal oncocytoma and chromophobe renal cell carcinoma in FNA and core needle biopsies. Cancer Cytopathol 123:298-305 (2015). PubMed: 25739652
- Rohde D et al. S100A1 is released from ischemic cardiomyocytes and signals myocardial damage via Toll-like receptor 4. EMBO Mol Med 6:778-94 (2014). PubMed: 24833748
- Holley M et al. Emx2 and early hair cell development in the mouse inner ear. Dev Biol 340:547-56 (2010). IHC-P ; Mouse . PubMed: 20152827