• Product name
  • Description
    Rabbit polyclonal to S100 alpha
  • Host species
  • Tested applications
    Suitable for: ELISA, IP, WB, IHC-P, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Dog, Human, Pig
  • Immunogen

    Full length native protein (purified) corresponding to Human S100 alpha. Purified S100-alpha protein from human pectoral muscle cells.

  • Positive control
    • Human muscle.



Our Abpromise guarantee covers the use of ab11428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 0.1 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa). Use at a concentration of 0.1 µg/ml. Detects a band of approximately 10 kDa, representing S100-alpha from human muscle. S100 protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane.
IHC-P Use a concentration of 1 µg/ml.
ICC 1/50 - 1/200.
ICC/IF 1/50 - 1/200.


  • Function
    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
  • Tissue specificity
    Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • Sequence similarities
    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Bpb antibody
    • NEF antibody
    • Protein S100-A1 antibody
    • S-100 protein alpha chain antibody
    • S-100 protein subunit alpha antibody
    • S100 alpha antibody
    • S100 Alpha Chain antibody
    • S100 antibody
    • S100 Beta Chain antibody
    • S100 Calcium Binding Protein A1 antibody
    • S100 Calcium Binding Protein B antibody
    • S100 Calcium Binding Protein Beta Neural antibody
    • S100 calcium-binding protein A1 antibody
    • S100 protein alpha polypeptide antibody
    • S100A antibody
    • s100a1 antibody
    • S10A1_HUMAN antibody
    see all


  • ab11428 (2µg/ml) staining S100 alpha in human breast tissue using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear and cytoplasmic compartment within the breast ductal regions
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab11428 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11428, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab11428 labelling S100 alpha (red) in HEK293T cells by Immunocytochemistry/Immunofluorescence. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were incubated with primary antibody (1:100 in blocking buffer) overnight at 4ºC. A fluorophore-conjugated goat anti-rabbit IgG (1:200) was used as the secondary antibody (1 hour at room temperature). Blue (DAPI) - nuclei. Images were taken at 40X magnification.

  • A Western blot of S100-alpha from human ovary extract (50µg) using ab11428 at 1/1000 dilution. A secondary goat anti rabbit HRP conjugate was used to visualise. 

    See Abreview


This product has been referenced in:
  • Zhang T  et al. Six1 is essential for differentiation and patterning of the mammalian auditory sensory epithelium. PLoS Genet 13:e1006967 (2017). Read more (PubMed: 28892484) »
  • Conner JR  et al. HNF1ß and S100A1 are useful biomarkers for distinguishing renal oncocytoma and chromophobe renal cell carcinoma in FNA and core needle biopsies. Cancer Cytopathol 123:298-305 (2015). Read more (PubMed: 25739652) »
See all 4 Publications for this product

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1-2 of 2 Abreviews

Western blot
Dog Tissue lysate - whole (Left ventricle)
Loading amount
3 µg
Left ventricle
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. sudhish mishra

Verified customer

Submitted Apr 29 2008

Western blot
Human Tissue lysate - whole (Ovary)
Loading amount
50 µg
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Mr. John Andersen

Verified customer

Submitted Jun 14 2006

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