Recombinant
RabMAb

Recombinant Anti-S100 alpha antibody [EPR5250] (ab133519)

Overview

  • Product name

    Anti-S100 alpha antibody [EPR5250]
    See all S100 alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR5250] to S100 alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the Human S100 alpha protein (UniProt ID: P23297).

  • Positive control

    • C2C12, Mouse heart and brain cell lysates; Human endometrial carcinoma and heart muscle tissue; Mouse heart tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 10 kDa.

For unpurified use at 1/1000 - 1/10000.

IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
    • Tissue specificity

      Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
    • Sequence similarities

      Belongs to the S-100 family.
      Contains 2 EF-hand domains.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • Bpb antibody
      • NEF antibody
      • Protein S100-A1 antibody
      • S-100 protein alpha chain antibody
      • S-100 protein subunit alpha antibody
      • S100 alpha antibody
      • S100 Alpha Chain antibody
      • S100 antibody
      • S100 Beta Chain antibody
      • S100 Calcium Binding Protein A1 antibody
      • S100 Calcium Binding Protein B antibody
      • S100 Calcium Binding Protein Beta Neural antibody
      • S100 calcium-binding protein A1 antibody
      • S100 protein alpha polypeptide antibody
      • S100A antibody
      • s100a1 antibody
      • S10A1_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebral cortex tissue sections labeling S100 alpha with purified ab133519 at 1:100 dilution (4.33 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

    • All lanes : Anti-S100 alpha antibody [EPR5250] (ab133519) at 1/1000 dilution (purified)

      Lane 1 : Anti-S100 alpha antibody [EPR5250] (ab133519) at 20 µg
      Lane 2 : Human fetal heart lysates at 15 µg

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 10 kDa



      Blocking and diluting buffer: 5% NBST/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebral cortex tissue sections labeling S100 alpha with purified ab133519 at 1:100 dilution (4.33 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skeletal muscle tissue sections labeling S100 alpha with purified ab133519 at 1:100 dilution (4.33 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human glioma tissue sections labeling S100 alpha with purified ab133519 at 1:100 dilution (4.33 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

    • Immunohistochemistry analysis of paraffin embedded Human endometrial carcinoma tissue labelling S100 alpha with unpurified ab133519 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-S100 alpha antibody [EPR5250] (ab133519) at 1/1000 dilution + Mouse heart lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 10 kDa
      Observed band size: 10 kDa



      5%NFDM/TBST

    • All lanes : Anti-S100 alpha antibody [EPR5250] (ab133519) at 1/1000 dilution

      Lane 1 : Mouse heart lysate
      Lane 2 : Mouse brain lysate
      Lane 3 : C2C12 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 10 kDa

    • Immunohistochemistry analysis of paraffin embedded Human heart muscle tissue labelling S100 alpha with unpurified  ab133519 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry analysis of paraffin embedded Mouse heart tissue labelling S100 alpha with unpurified  ab133519 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab133519 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Heart)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate 70C 2hr
    Permeabilization
    No
    Specification
    Heart
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Nov 29 2017

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