Product nameAnti-S100 alpha antibody [EPR5251]
See all S100 alpha primary antibodies
DescriptionRabbit monoclonal [EPR5251] to S100 alpha
Tested applicationsSuitable for: WB, IP, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human S100 alpha aa 1-100. The exact sequence is proprietary.
- WB: kidney, C2C12, mouse kidney, Human brain, HT-1080 and mouse heart lysate IF: HeLa cells
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.00 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 11 kDa.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
FunctionWeakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
Tissue specificityHighly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
Sequence similaritiesBelongs to the S-100 family.
Contains 2 EF-hand domains.
- Information by UniProt
- Bpb antibody
- NEF antibody
- Protein S100-A1 antibody
All lanes : Anti-S100 alpha antibody [EPR5251] (ab109384) at 1/1000 dilution
Lane 1 : Rat kidney lysate
Lane 2 : C2C12 lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Human brain lysate
Lane 5 : HT-1080 lysate
Lane 6 : Mouse heart lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 11 kDa
ab109384, at a 1/100 dilution staining S100 alpha in HeLa cells
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab109384 has not yet been referenced specifically in any publications.