Recombinant Anti-S100 alpha antibody [EPR5251] (ab109384)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5251] to S100 alpha
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-S100 alpha antibody [EPR5251]
See all S100 alpha primary antibodies -
Description
Rabbit monoclonal [EPR5251] to S100 alpha -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC/IF HumanWB Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: kidney, C2C12, mouse kidney, Human brain, HT-1080 and mouse heart lysate IF: HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.00 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5251 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
ICC/IF |
Human
|
WB |
Rat
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
|
|
ICC/IF |
1/100 - 1/250.
|
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 11 kDa. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. -
Tissue specificity
Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. -
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 6271 Human
- Entrez Gene: 20193 Mouse
- Entrez Gene: 295214 Rat
- Omim: 176940 Human
- SwissProt: P23297 Human
- SwissProt: P56565 Mouse
- SwissProt: P35467 Rat
- Unigene: 515715 Human
see all -
Alternative names
- Bpb antibody
- NEF antibody
- Protein S100-A1 antibody
see all
Images
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All lanes : Anti-S100 alpha antibody [EPR5251] (ab109384) at 1/1000 dilution
Lane 1 : Rat kidney lysate
Lane 2 : C2C12 lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Human brain lysate
Lane 5 : HT-1080 lysate
Lane 6 : Mouse heart lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 11 kDa -
ab109384, at a 1/100 dilution staining S100 alpha in HeLa cells
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab109384 has not yet been referenced specifically in any publications.