Recombinant
RabMAb

Recombinant Anti-S100 alpha antibody [EPR5251] (ab109384)

Submit a review Submit a question

Overview

  • Product name

    Anti-S100 alpha antibody [EPR5251]
    See all S100 alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR5251] to S100 alpha

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human S100 alpha aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: kidney, C2C12, mouse kidney, Human brain, HT-1080 and mouse heart lysate IF: HeLa cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab109384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.

    • Tissue specificity

      Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.

    • Sequence similarities

      Belongs to the S-100 family.
      Contains 2 EF-hand domains.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Bpb antibody
      • NEF antibody
      • Protein S100-A1 antibody
      • S-100 protein alpha chain antibody
      • S-100 protein subunit alpha antibody
      • S100 alpha antibody
      • S100 Alpha Chain antibody
      • S100 antibody
      • S100 Beta Chain antibody
      • S100 Calcium Binding Protein A1 antibody
      • S100 Calcium Binding Protein B antibody
      • S100 Calcium Binding Protein Beta Neural antibody
      • S100 calcium-binding protein A1 antibody
      • S100 protein alpha polypeptide antibody
      • S100A antibody
      • s100a1 antibody
      • S10A1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-S100 alpha antibody [EPR5251] (ab109384)
      Western blot - Anti-S100 alpha antibody [EPR5251] (ab109384)
      All lanes : Anti-S100 alpha antibody [EPR5251] (ab109384) at 1/1000 dilution

      Lane 1 : Rat kidney lysate
      Lane 2 : C2C12 lysate
      Lane 3 : Mouse kidney lysate
      Lane 4 : Human brain lysate
      Lane 5 : HT-1080 lysate
      Lane 6 : Mouse heart lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 11 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha antibody [EPR5251] (ab109384)
      Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha antibody [EPR5251] (ab109384)
      ab109384, at a 1/100 dilution staining S100 alpha in HeLa cells
    • Other - Anti-S100 alpha antibody [EPR5251] (ab109384)
      Other - Anti-S100 alpha antibody [EPR5251] (ab109384)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab109384 has not yet been referenced specifically in any publications.

    Publishing research using ab109384? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab109384.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com