RecombinantRabMAb

Recombinant Anti-S100 alpha antibody [EPR5251] (ab109384)

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Western blot - Anti-S100 alpha antibody [EPR5251] (ab109384)
  • Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha antibody [EPR5251] (ab109384)
  • OI-RD Scanning - Anti-S100 alpha antibody [EPR5251] (ab109384)
  • Anti-S100 alpha antibody [EPR5251] (ab109384)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5251] to S100 alpha
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-S100 alpha antibody [EPR5251]
    See all S100 alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR5251] to S100 alpha

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: kidney, C2C12, mouse kidney, Human brain, HT-1080 and mouse heart lysate IF: HeLa cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Rat
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
ICC/IF
1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.

    • Tissue specificity

      Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.

    • Sequence similarities

      Belongs to the S-100 family.
      Contains 2 EF-hand domains.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Bpb antibody
      • NEF antibody
      • Protein S100-A1 antibody
      • S-100 protein alpha chain antibody
      • S-100 protein subunit alpha antibody
      • S100 alpha antibody
      • S100 Alpha Chain antibody
      • S100 antibody
      • S100 Beta Chain antibody
      • S100 Calcium Binding Protein A1 antibody
      • S100 Calcium Binding Protein B antibody
      • S100 Calcium Binding Protein Beta Neural antibody
      • S100 calcium-binding protein A1 antibody
      • S100 protein alpha polypeptide antibody
      • S100A antibody
      • s100a1 antibody
      • S10A1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-S100 alpha antibody [EPR5251] (ab109384)
      Western blot - Anti-S100 alpha antibody [EPR5251] (ab109384)
      All lanes : Anti-S100 alpha antibody [EPR5251] (ab109384) at 1/1000 dilution

      Lane 1 : Rat kidney lysate
      Lane 2 : C2C12 lysate
      Lane 3 : Mouse kidney lysate
      Lane 4 : Human brain lysate
      Lane 5 : HT-1080 lysate
      Lane 6 : Mouse heart lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 11 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha antibody [EPR5251] (ab109384)
      Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha antibody [EPR5251] (ab109384)
      ab109384, at a 1/100 dilution staining S100 alpha in HeLa cells
    • OI-RD Scanning - Anti-S100 alpha antibody [EPR5251] (ab109384)
      OI-RD Scanning - Anti-S100 alpha antibody [EPR5251] (ab109384)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-S100 alpha antibody [EPR5251] (ab109384)
      Anti-S100 alpha antibody [EPR5251] (ab109384)

    References (0)

    Publishing research using ab109384? Please let us know so that we can cite the reference in this datasheet.

    ab109384 has not yet been referenced specifically in any publications.

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