Anti-S100 alpha antibody [EPR5251] (ab109384)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-S100 alpha antibody [EPR5251]
See all S100 alpha primary antibodies -
DescriptionRabbit monoclonal [EPR5251] to S100 alpha
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human S100 alpha aa 1-100. The exact sequence is proprietary.
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Positive control
- WB: kidney, C2C12, mouse kidney, Human brain, HT-1080 and mouse heart lysate IF: HeLa cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Dissociation constant (KD)KD = 5.00 x 10 -11 M Learn more about KD
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR5251
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab109384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 11 kDa. | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| ICC/IF | 1/100 - 1/250. |
Target
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FunctionWeakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
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Tissue specificityHighly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
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Sequence similaritiesBelongs to the S-100 family.
Contains 2 EF-hand domains. -
Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 6271 Human
- Entrez Gene: 20193 Mouse
- Entrez Gene: 295214 Rat
- Omim: 176940 Human
- SwissProt: P23297 Human
- SwissProt: P56565 Mouse
- SwissProt: P35467 Rat
- Unigene: 515715 Human
see all -
Alternative names
- Bpb antibody
- NEF antibody
- Protein S100-A1 antibody
see all
Images
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![Western blot - Anti-S100 alpha antibody [EPR5251] (ab109384)](https://www.abcam.com/ps/products/109/ab109384/Images/S100-alpha-Primary-antibodies-ab109384-1.jpg)
Western blot - Anti-S100 alpha antibody [EPR5251] (ab109384)All lanes : Anti-S100 alpha antibody [EPR5251] (ab109384) at 1/1000 dilution
Lane 1 : Rat kidney lysate
Lane 2 : C2C12 lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Human brain lysate
Lane 5 : HT-1080 lysate
Lane 6 : Mouse heart lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 11 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha antibody [EPR5251] (ab109384)](https://www.abcam.com/ps/products/109/ab109384/Images/S100-alpha-Primary-antibodies-ab109384-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha antibody [EPR5251] (ab109384)ab109384, at a 1/100 dilution staining S100 alpha in HeLa cells -
![Other - Anti-S100 alpha antibody [EPR5251] (ab109384)](https://www.abcam.com/ps/products/109/ab109384/Images/S100-alpha-Primary-antibodies-ab109384-3.jpg)
Other - Anti-S100 alpha antibody [EPR5251] (ab109384)
Protocols
References
ab109384 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"