Full length native protein (purified) (Human brain).
Our Abpromise guarantee covers the use of ab14849 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.|
|IHC-P||1/3000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See Abreview.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 28955220|
ab14849 staining S100 in human melanoma cultured cells by ICC (Immunocytochemistry). Cells were fixed with 4% paraformaldehyde in PBS, permeabilized with 0.2% Triton X-100 for 15 minutes and blocked for 30 minutes at 21°C. Samples were incubated with primary antibody (at concentrations listed above) for 15 hours at 4°C. A HRP-conjugated anti-mouse IgG polyclonal was used as the secondary antibody.
ab14849 staining S100 in the human Melanoma cell line 1205Lu xenograph tissue sections by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde, permeabilized with 0.1% Triton X100 and blocked with a commercial block solution for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris EDTA at pH 9. The sample was incubated with primary antibody (1/300 in blocking solution) at 4°C for 18 hours. A biotin-conjugated goat polyclonal to mouse IgG (1/50) was used as secondary antibody.
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