Overview

  • Product name
    Anti-S100 antibody [4C4.9]
    See all S100 primary antibodies
  • Description
    Mouse monoclonal [4C4.9] to S100
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Tissue, cells or virus. purified bovine brain S-100 protein

  • Positive control
    • Melanoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab4066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23624931
IHC-P 1/10 - 1/50. Antigen retrieval is not essential but may optimise staining.
IHC-Fr 1/200 - 1/500.
WB Use at an assay dependent concentration.

Target

  • Function
    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
  • Tissue specificity
    Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • Sequence similarities
    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Bpb antibody
    • NEF antibody
    • Protein S100-A1 antibody
    • S-100 protein alpha chain antibody
    • S-100 protein subunit alpha antibody
    • S100 alpha antibody
    • S100 beta antibody
    • S100 calcium binding protein A1 antibody
    • S100 calcium binding protein B antibody
    • S100 calcium-binding protein A1 antibody
    • S100 protein alpha polypeptide antibody
    • S100A antibody
    • s100a1 antibody
    • S100B antibody
    • S100beta antibody
    • S10A1_HUMAN antibody
    see all

Images

  • Immunohistochemistry (Frozen sections) - Anti-S100 antibody [4C4.9] (ab4066)
    Immunohistochemistry (Frozen sections) - Anti-S100 antibody [4C4.9] (ab4066)This image is courtesy of an Abreview submitted by Nancy Nutile-McMenemy
    ab4066 at a 1/200 dilution staining rat spinal cord tissue sections by Immunohistochemistry (Frozen sections). Rats were transcardially perfused with 4% PFA, the tissue was post fixed 1 hour in 4% PFA then 30% sucrose for three days. 20um sections were cut with cryostat. The antibody was incubated with the tissue sections for 18 hours and then bound antibody was detected using an Alexa Fluor ® 555 conjugated goat polyclonal antibody.

    See Abreview

  • Western blot - Anti-S100 antibody [4C4.9] (ab4066)
    Western blot - Anti-S100 antibody [4C4.9] (ab4066)This image is courtesy of an Abreview submitted by Ms Nancy Nutile-McMenemy
    All lanes : Anti-S100 antibody [4C4.9] (ab4066) at 1/200 dilution

    Lanes 1-3 : 40ug Rat spinal cord homogenate
    Lanes 4-6 : 40 ug Mouse spinal cord homogenate
    Lanes 7-8 : 40 ug Rat brain homogenate

    Secondary
    All lanes : HRP conjugated goat anti-mouse

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 15,18,21 kDa (why is the actual band size different from the predicted?)


    Exposure time: 2 minutes


    The extra band at 18kDa may be a result of the protein's thermolabile properties. The band at 21 kDa maybe a dimer of s100a and s100b and the 10/15 kDa may be the subunits. However, there are also references to suggest these may be immunoreactive proteins with differing molecular weights depending on proliferative or differentiated state of the cell (Anat Embryol (Berl). 1997 Nov;196(5):403-16).

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
    ab4066 staining human melanoma by IHC-P.

References

This product has been referenced in:
  • Breun M  et al. CXCR4: A new player in vestibular schwannoma pathogenesis. Oncotarget 9:9940-9950 (2018). Read more (PubMed: 29515781) »
  • Ohtake Y  et al. Axonal Activation of the Unfolded Protein Response Promotes Axonal Regeneration Following Peripheral Nerve Injury. Neuroscience 375:34-48 (2018). ICC/IF ; Mouse . Read more (PubMed: 29438804) »

See all 29 Publications for this product

Publishing research using ab4066? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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21 reviews or Q&A

10
Votes: Highest First

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 antibody [4C4.9]

 Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (melanoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrite buffer, pH6.0
Permeabilization
No
Specification
melanoma
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
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Verified customer

Submitted Aug 19 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 antibody [4C4.9]

 Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Antigen retrieval step
Other
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde
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Submitted Dec 14 2015

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-S100 antibody [4C4.9]

 Inconclusive
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain (Hippocampus))
Antigen retrieval step
None
Permeabilization
Yes - 0.1%TritonX-100/PBS
Specification
Brain (Hippocampus)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.5, 2 · Temperature: RT°C
Fixative
Paraformaldehyde
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Verified customer

Submitted May 14 2015

IHC-FoFr with rat peripheral nerve
sees no staining
fix: 4% PFA perfused, postfixed with PFA
no AR
Ab: 1/100 o/n 4 degree; 1/50 o/n RT
block: BSA + goat serum
2nd: goat anti-mouse, OK

suggested: try an enzymatic AR step


As we discussed, trying an enzymatic antigen retrieval step could help with the signal.

If not, please let me know.

As for the protocol on the datasheet - this was added most likely from a collaborator or customer. Thus, these mu...

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Dear Sir or Madam,

could you please also provide me with the concentration of your product Anti-S100 antibody [4C4.9] - Astrocyte Marker (ab4066)?

Best Regards,

Thank you for your interest in our products.

The unit size of this antibody (ab4066) is 500 µl and the concentration is not determined since it is not affinity purified - only IgG fraction.

If I can assist further, please do n...

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Thank you for sending the replacement antibody. This one worked very well both in vitro using human induced pluripotent stem cells and in vivo using mouse spinal cord sections.

That's great news! Thanks so much for letting us know.

Please feel free to contact me if you need anything further.

I have used this antibody on astrocytes grown in culture in ICC and mousespinal cord via IHC-Fr. In neither application did I see any signal using 1:200 dilution of the antibody; using another antibody to an astrocyte marker protein, GFAP, is was able ...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1080427.

To check the status of the order please con...

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Immunohistochemistry (Frozen sections) abreview for Anti-S100 antibody [4C4.9] - Astrocyte Marker

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Acetone
Permeabilization
Yes - 0,1% Saponin / PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Read More
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Abcam user community

Verified customer

Submitted Feb 27 2012

Thanks for the antibody. It works fine the immunostaining. But I still can not get any signal in western blot, although it was reported that it should work in western blot.



Do you have any other recommendations for western blot?

Thank you for your reply. After reviewing the customer feedback for ab4066, it appears that the customers who had obtained good WB results were using BSA as a blocking agent. The feedback we had from a customer using 5% milk was that their WB was unsuc...

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I am trying to do Western Blot and ICC for s100, a marker for astrocytes. The antibody which I got from Abcam is ab4066. I did not get any signal for WB and very weak signal for ICC. So I wonder what is the problem. If I still can not get any signal af...

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Thank you for contacting us. I am sorry that ab4066 was not producing satisfactory signal in ICC and WB. Our antibody ab868 is rated highly for use in ICC and WB on human, mouse, rat, cow, and pig samples. If you could please provide some additional de...

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1-10 of 21 Abreviews or Q&A

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