Anti-S100 antibody [4C4.9] (ab4066)

As we discussed, trying an enzymatic antigen retrieval step could help with the signal.

If not, please let me know.

As for the protocol on the datasheet - this was added most likely from a collaborator or customer. Thus, these must be the conditions that were used by them. I still would suggest to do the overnight incubation at 4 degree as this makes more sense.

Thank you for your interest in our products.

The unit size of this antibody (ab4066) is 500 µl and the concentration is not determined since it is not affinity purified - only IgG fraction.

If I can assist further, please do not hesitate to contact me.

That's great news! Thanks so much for letting us know.

Please feel free to contact me if you need anything further.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1080427.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you for your reply. After reviewing the customer feedback for ab4066, it appears that the customers who had obtained good WB results were using BSA as a blocking agent. The feedback we had from a customer using 5% milk was that their WB was unsuccessful. It is possible that this antibody may be sensitive to milk and may produce better results when using BSA.

For both ab4066 andab868, you could also try increasing the primary antibody concentration to the range 1:200-1:500 or loading more protein (30-50ug per lane). I hope this helps, please let me know if you need any further assistance.

Thank you for contacting us. I am sorry that ab4066 was not producing satisfactory signal in ICC and WB. Our antibody ab868 is rated highly for use in ICC and WB on human, mouse, rat, cow, and pig samples. If you could please provide some additional details about the protocol and samples you are using, I would be happy to assist you further. If ab4066 is not working as stated on the datasheet and you have purchased it in the past six months, I would be happy to send you ab868 as a free of charge replacement. Could you please provide the following information? 1) What is your original order or PO number? 2) What species and sample type were you testing? How were the cells / tissuefixed or lysed? 3) What permeabilization and/ or blocking agents were used? 4) Could you please tell me the dilution and incubation times for the primary and secondary antibodies? 5) It would be very helpful if you could please provide an image of your WB or ICC.