Anti-S100 antibody [4C4.9] (ab4066)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)](https://www.abcam.com/ps/products/4/ab4066/Images/ab4066-448306-anti-s100-antibody-immunohistochemistry-Melanoma2-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)")
Key features and details
- Mouse monoclonal [4C4.9] to S100
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
-
Product name
Anti-S100 antibody [4C4.9]
See all S100 primary antibodies -
Description
Mouse monoclonal [4C4.9] to S100 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein. Purified bovine brain S-100 protein
-
Positive control
- IHC-P: Human Melanoma and skin tissue.
-
General notes
This product was changed from ascites to tissue culture supernatant on 29/01/2021. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituent: Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
4C4.9 -
Isotype
IgG2a -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (4) |
1/10 - 1/50. Antigen retrieval is not essential but may optimise staining.
|
| Notes |
|---|
|
IHC-P
1/10 - 1/50. Antigen retrieval is not essential but may optimise staining. |
Target
-
Function
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. -
Tissue specificity
Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. -
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 6271 Human
- Omim: 176940 Human
- SwissProt: P23297 Human
- Unigene: 515715 Human
-
Alternative names
- Bpb antibody
- NEF antibody
- Protein S100-A1 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
Immunohistochemistry of Formalin fixed paraffin embedded human melanoma tissue sections labeling S100 with ab4066 at 1/100. (x10 magnification).
This image was generated using the ascites version of the product.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)](https://www.abcam.com/ps/products/4/ab4066/Images/ab4066-448305-anti-s100-antibody-immunohistochemistry-Melanoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)Immunohistochemistry of Formalin fixed paraffin embedded human melanoma tissue sections labeling S100 with ab4066 at 1/100. (x10 magnification).
This image was generated using the ascites version of the product.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)](https://www.abcam.com/ps/products/4/ab4066/Images/ab4066-448304-anti-s100-antibody-immunohistochemistry-skin-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)Immunohistochemistry of Formalin fixed paraffin embedded human skin labeling S100 with ab4066.This image was generated using the ascites version of the product.
Datasheets and documents
-
SDS download
-
Datasheet download
References (66)
ab4066 has been referenced in 66 publications.
- Qu H et al. Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease. Diabetes 70:1372-1387 (2021). PubMed: 33741719
- Hernández-Díaz N et al. Parallel evolution of placental calcium transfer in the lizard Mabuya and eutherian mammals. J Exp Biol 224:N/A (2021). PubMed: 33568441
- Wang Y et al. Betacellulin regulates peripheral nerve regeneration by affecting Schwann cell migration and axon elongation. Mol Med 27:27 (2021). PubMed: 33794764
- Omiya H et al. BMP signaling suppresses Gemc1 expression and ependymal differentiation of mouse telencephalic progenitors. Sci Rep 11:613 (2021). PubMed: 33436697
- Rinwa P et al. Demise of nociceptive Schwann cells causes nerve retraction and pain hyperalgesia. Pain 162:1816-1827 (2021). PubMed: 33979318