Anti-S100 antibody [4C4.9] (ab4066)
- Datasheet
- References (31)
- Protocols
Overview
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Product nameAnti-S100 antibody [4C4.9]
See all S100 primary antibodies -
DescriptionMouse monoclonal [4C4.9] to S100
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Host speciesMouse
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Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, WBmore details
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Species reactivityReacts with: Mouse, Rat, Cow, Human
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Immunogen
Tissue, cells or virus. purified bovine brain S-100 protein
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Positive control
- Melanoma.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: Ascites, 1% BSA -
Concentration information loading...
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PurityProtein G purified
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ClonalityMonoclonal
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Clone number4C4.9
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IsotypeIgG2a
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Research areas
Associated products
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Alternative Products
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab4066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | Use at an assay dependent concentration. PubMed: 23624931 | |
IHC-P | 1/10 - 1/50. Antigen retrieval is not essential but may optimise staining. | |
IHC-Fr | 1/200 - 1/500. | |
WB | Use at an assay dependent concentration. |
Target
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FunctionWeakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
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Tissue specificityHighly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
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Sequence similaritiesBelongs to the S-100 family.
Contains 2 EF-hand domains. -
Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 431787 Cow
- Entrez Gene: 6271 Human
- Entrez Gene: 20193 Mouse
- Entrez Gene: 295214 Rat
- Omim: 176940 Human
- SwissProt: P02639 Cow
- SwissProt: P23297 Human
- SwissProt: P56565 Mouse
see all -
Alternative names
- Bpb antibody
- NEF antibody
- Protein S100-A1 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-S100 antibody [4C4.9] (ab4066)This image is courtesy of an Abreview submitted by Nancy Nutile-McMenemyab4066 at a 1/200 dilution staining rat spinal cord tissue sections by Immunohistochemistry (Frozen sections). Rats were transcardially perfused with 4% PFA, the tissue was post fixed 1 hour in 4% PFA then 30% sucrose for three days. 20um sections were cut with cryostat. The antibody was incubated with the tissue sections for 18 hours and then bound antibody was detected using an Alexa Fluor ® 555 conjugated goat polyclonal antibody.
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Western blot - Anti-S100 antibody [4C4.9] (ab4066)This image is courtesy of an Abreview submitted by Ms Nancy Nutile-McMenemyAll lanes : Anti-S100 antibody [4C4.9] (ab4066) at 1/200 dilution
Lanes 1-3 : 40ug Rat spinal cord homogenate
Lanes 4-6 : 40 ug Mouse spinal cord homogenate
Lanes 7-8 : 40 ug Rat brain homogenate
Secondary
All lanes : HRP conjugated goat anti-mouse
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 15,18,21 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
The extra band at 18kDa may be a result of the protein's thermolabile properties. The band at 21 kDa maybe a dimer of s100a and s100b and the 10/15 kDa may be the subunits. However, there are also references to suggest these may be immunoreactive proteins with differing molecular weights depending on proliferative or differentiated state of the cell (Anat Embryol (Berl). 1997 Nov;196(5):403-16). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)ab4066 staining human melanoma by IHC-P.
Protocols
References
This product has been referenced in:
- Lu Y et al. Fibroblast growth factor 21 facilitates peripheral nerve regeneration through suppressing oxidative damage and autophagic cell death. J Cell Mol Med 23:497-511 (2019). Read more (PubMed: 30450828) »
- Breun M et al. CXCR4: A new player in vestibular schwannoma pathogenesis. Oncotarget 9:9940-9950 (2018). Read more (PubMed: 29515781) »