Anti-S100 antibody [4C4.9] (ab4066)

Mouse monoclonal S100 antibody [4C4.9]. Validated in WB, IHC, ICC/IF and tested in Mouse, Rat, Cow, Human. Cited in 31 publication(s). Independently reviewed in 10 review(s).

Overview

  • Product name

    Anti-S100 antibody [4C4.9]
    See all S100 primary antibodies
  • Description

    Mouse monoclonal [4C4.9] to S100
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, IHC-P, IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Tissue, cells or virus. purified bovine brain S-100 protein

  • Positive control

    • Melanoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab4066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23624931
IHC-P 1/10 - 1/50. Antigen retrieval is not essential but may optimise staining.
IHC-Fr 1/200 - 1/500.
WB Use at an assay dependent concentration.

Target

  • Function

    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
  • Tissue specificity

    Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • Sequence similarities

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Bpb antibody
    • NEF antibody
    • Protein S100-A1 antibody
    • S-100 protein alpha chain antibody
    • S-100 protein subunit alpha antibody
    • S100 alpha antibody
    • S100 beta antibody
    • S100 calcium binding protein A1 antibody
    • S100 calcium binding protein B antibody
    • S100 calcium-binding protein A1 antibody
    • S100 protein alpha polypeptide antibody
    • S100A antibody
    • s100a1 antibody
    • S100B antibody
    • S100beta antibody
    • S10A1_HUMAN antibody
    see all

Images

  • ab4066 at a 1/200 dilution staining rat spinal cord tissue sections by Immunohistochemistry (Frozen sections). Rats were transcardially perfused with 4% PFA, the tissue was post fixed 1 hour in 4% PFA then 30% sucrose for three days. 20um sections were cut with cryostat. The antibody was incubated with the tissue sections for 18 hours and then bound antibody was detected using an Alexa Fluor ® 555 conjugated goat polyclonal antibody.

    See Abreview

  • All lanes : Anti-S100 antibody [4C4.9] (ab4066) at 1/200 dilution

    Lanes 1-3 : 40ug Rat spinal cord homogenate
    Lanes 4-6 : 40 ug Mouse spinal cord homogenate
    Lanes 7-8 : 40 ug Rat brain homogenate

    Secondary
    All lanes : HRP conjugated goat anti-mouse

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 15,18,21 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes


    The extra band at 18kDa may be a result of the protein's thermolabile properties. The band at 21 kDa maybe a dimer of s100a and s100b and the 10/15 kDa may be the subunits. However, there are also references to suggest these may be immunoreactive proteins with differing molecular weights depending on proliferative or differentiated state of the cell (Anat Embryol (Berl). 1997 Nov;196(5):403-16).

    See Abreview

  • ab4066 staining human melanoma by IHC-P.

References

This product has been referenced in:

  • Lu Y  et al. Fibroblast growth factor 21 facilitates peripheral nerve regeneration through suppressing oxidative damage and autophagic cell death. J Cell Mol Med 23:497-511 (2019). Read more (PubMed: 30450828) »
  • Passipieri JA  et al. Adipose Stem Cells Enhance Nerve Regeneration and Muscle Function In A Peroneal Nerve Ablation Model. Tissue Eng Part A N/A:N/A (2019). Read more (PubMed: 30760135) »
See all 39 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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1-10 of 10 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (melanoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrite buffer, pH6.0
Permeabilization
No
Specification
melanoma
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 19 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Antigen retrieval step
Other
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Dec 14 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain (Hippocampus))
Antigen retrieval step
None
Permeabilization
Yes - 0.1%TritonX-100/PBS
Specification
Brain (Hippocampus)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.5, 2 · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 14 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Acetone
Permeabilization
Yes - 0,1% Saponin / PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 27 2012

Application
Western blot
Sample
Rat Tissue lysate - whole (Spinal Cord homogenate)
Loading amount
30 µg
Specification
Spinal Cord homogenate
Treatment
Thoracotomy or L5 nerve transection
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted May 30 2008

Application
Immunocytochemistry
Sample
Rat Cultured Cells (rat Schwann cells)
Specification
rat Schwann cells
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 18°C

Abcam user community

Verified customer

Submitted May 15 2008

Application
Western blot
Sample
Human Cell lysate - other (Human cell line (normal and cancer))
Loading amount
40 µg
Specification
Human cell line (normal and cancer)
Gel Running Conditions
Reduced Denaturing (4-15% Tris HCL)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 26 2008

Application
Western blot
Sample
Rat Tissue lysate - whole (spinal cord)
Loading amount
40 µg
Specification
spinal cord
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Ms. Nancy Nutile-McMenemy

Verified customer

Submitted Sep 18 2006

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Spinal Cord)
Specification
Spinal Cord
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Ms. Nancy Nutile-McMenemy

Verified customer

Submitted Jun 21 2006

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (rat pup cortical preps)
Specification
rat pup cortical preps
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Ms. Nancy Nutile-McMenemy

Verified customer

Submitted Jun 21 2006

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