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IHC-FoFr with rat peripheral nerve
sees no staining
fix: 4% PFA perfused, postfixed with PFA
Ab: 1/100 o/n 4 degree; 1/50 o/n RT
block: BSA + goat serum
2nd: goat anti-mouse, OK
suggested: try an enzymatic AR step
Asked on Feb 21 2013
As we discussed, trying an enzymatic antigen retrieval step could help with the signal.
If not, please let me know.
As for the protocol on the datasheet - this was added most likely from a collaborator or customer. Thus, these must be the conditions that were used by them. I still would suggest to do the overnight incubation at 4 degree as this makes more sense.
Answered on Feb 21 2013