Immunocytochemistry abreview for Anti-S100 antibody [4C4.9] - Astrocyte Marker

Excellent
Abreviews
Application
Immunocytochemistry
Sample
Rat Cultured Cells (rat Schwann cells)
Specification
rat Schwann cells
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 18°C

Other product details

Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 18°C

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 594-goat anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/500

Additional data

Additional Notes
This antibody works best with Formaldehyde/Paraformaldehyde fixation, but not with the Acetone. The best working dilution for cell culture in my experiments determined to be 1/100-1/200.

Abcam user community

Verified customer

Submitted May 15 2008

Sign up