Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-S100 antibody [4C4.9]

Inconclusive
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain (Hippocampus))
Antigen retrieval step
None
Permeabilization
Yes - 0.1%TritonX-100/PBS
Specification
Brain (Hippocampus)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.5, 2 · Temperature: RT°C
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Diluent: 0.5%BSA/PBS
Dilution
1/100

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: 568 goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568

Additional data

Abcam response
Thank you for your valuable feedback. Unfortunately, we have not tested in IHC-FoFr and can not guarantee in the application.

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Submitted May 14 2015

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