Key features and details
- Mouse monoclonal [6G1] to S100 (HRP)
- Suitable for: ELISA, Sandwich ELISA, Purification, WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Product nameAnti-S100 antibody [6G1] (HRP)
See all S100 primary antibodies
DescriptionMouse monoclonal [6G1] to S100 (HRP)
Tested applicationsSuitable for: ELISA, Sandwich ELISA, Purification, WBmore details
Species reactivityReacts with: Human
Human S-100 protein
This product was changed from ascites to tissue culture supernatant on 29th April 2020. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Concentration varies from lot to lot and can be provided on request.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Preservative: 0.05% Proclin 300
Constituents: 0.8% Sodium chloride, 0.02% Potassium chloride, 0.024% Monobasic dihydrogen potassium phosphate, 0.18% Dibasic monohydrogen sodium phosphate
Concentration information loading...
Purification notesPurified from ascites.
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab10203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|Sandwich ELISA||Use at an assay dependent dilution. Use as Detection antibody. Sensitive to EDTA or other bivalent-binding agents. Better performance can be obtained in presence of 5mM CaCl2 in thses assay buffers. Can be used as detection antibody in conjunction with ab8330 and ab8334.|
|Purification||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution.|
FunctionWeakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
Tissue specificityHighly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
Sequence similaritiesBelongs to the S-100 family.
Contains 2 EF-hand domains.
- Information by UniProt
- Bpb antibody
- NEF antibody
- Protein S100-A1 antibody
S-100 calibration curve, from one step assay in streptavidin coated plates. Ab8330 [200 ng/well] was used as capture and Eu- labelled ab10203 [200 ng/well] as detection. Antigen used was S-100 protein from the human brain. Incubated 20 minutes at 20°C.
Interaction of several monoclonal antibodies with purified S100 protein (1
g) from human brain in WB, after native gel electrophoresis using the Ornstein-Davis system. Lane 1 = ab14849, Lane 2 = ab8330, ab Lane 3 = ab10203, Lane 4 = ab8334. The doublet band appears at about 20-21 kD, Interaction of several monoclonal antibodies with purified S100 protein (1 µg) from human brain in WB, after native gel electrophoresis using the Ornstein-Davis system. Lane 1 = ab14849, Lane 2 = ab8330, ab Lane 3 = ab10203, Lane 4 = ab8334. The doublet band appears at about 20-21 kD, µ
ab10203 has been referenced in 1 publication.
- Mathew AS et al. Detection of a Traumatic Brain Injury Biomarker at the 10 fg/mL Level. Mol Diagn Ther 22:729-735 (2018). PubMed: 30377977