Key features and details
- Rabbit polyclonal to S100
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG
Product nameAnti-S100 antibody
See all S100 primary antibodies
DescriptionRabbit polyclonal to S100
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human
Predicted to work with: Dog, Non human primates, Mammals
Full length native protein (purified): MGSELETAME TLINVFHAHS GKEGDKYKLS KKELKELLQT ELSGFLDAQK DADAVDKVMK ELDENGDGEV DFQEYVVLVA ALTVACNNFF WENS, corresponding to amino acids 1-94 of Cow S100 (alpha chain). A mixture of alpha alpha homodimers and alpha beta heterodimers was used.
- IHC-P: human brain astrocyte tumor tissue and Mouse brain tissue. WB: rat brain whole cell lysate.
General notesPlease note that lot 296545 is supplied at 0.5mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityProtein A purified
Purification notesThis antibody was purified from monospecific antiserum.
Our Abpromise guarantee covers the use of ab34686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.67 - 10 µg/ml. Detects a band of approximately 11 kDa.|
|IHC-P||Use a concentration of 2.5 - 25 µg/ml.|
FunctionWeakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
Tissue specificityHighly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
Sequence similaritiesBelongs to the S-100 family.
Contains 2 EF-hand domains.
- Information by UniProt
- Bpb antibody
- NEF antibody
- Protein S100-A1 antibody
ab34686 staining S100 in Rat nerve tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a 10mM citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/250 in PBS + 1x casein) for 1 hour at 25°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
All lanes : Anti-S100 antibody (ab34686) at 1/1000 dilution
Lane 1 : Bovine S100 monomer at 0.1 µg
Lane 2 : S100 from rat brain lysate at 35 µg
All lanes : IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX
Observed band size: 11 kDa why is the actual band size different from the predicted?
Ab34686, at a 1/500 dilution, staining S100 in mouse brain tissue by immunohistochemistry. The tissue was formalin fixed and embedded in paraffin. Dark nuclear staining is observed within basket cells located near the Purkinje cells in the cerebellum.
Ab34686, at 1/500 dilution, staining S100 in formalin fixed and paraffin embedded human brain astrocyte tumor tissue by immunohistochemistry.
ab34686 has been referenced in 2 publications.
- Gao L et al. Targeted next-generation sequencing of dedifferentiated chondrosarcoma in the skull base reveals combined TP53 and PTEN mutations with increased proliferation index, an implication for pathogenesis. Oncotarget 7:43557-43569 (2016). PubMed: 27248819
- Hsu M & Stevenson FF Reduction in muscular motility by selective focused cold therapy: a preclinical study. J Neural Transm 121:15-20 (2014). IHC-P ; Rat . PubMed: 23917804