Anti-S100 antibody (Biotin) (ab48653)

Submit a review Q&A (2)

Overview

  • Product name
    Anti-S100 antibody (Biotin)
    See all S100 primary antibodies
  • Description
    Rabbit polyclonal to S100 (Biotin)
  • Host species
    Rabbit
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: WB, ELISA, IP, RIAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein purified from human plasma

Properties

Applications

Our Abpromise guarantee covers the use of ab48653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.

Target

  • Function
    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
  • Tissue specificity
    Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • Sequence similarities
    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    • Alternative names
      • Bpb antibody
      • NEF antibody
      • Protein S100-A1 antibody
      • S-100 protein alpha chain antibody
      • S-100 protein subunit alpha antibody
      • S100 alpha antibody
      • S100 beta antibody
      • S100 calcium binding protein A1 antibody
      • S100 calcium binding protein B antibody
      • S100 calcium-binding protein A1 antibody
      • S100 protein alpha polypeptide antibody
      • S100A antibody
      • s100a1 antibody
      • S100B antibody
      • S100beta antibody
      • S10A1_HUMAN antibody
      see all

    References

    ab48653 has not yet been referenced specifically in any publications.

    Publishing research using ab48653? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    Customer Services and Scientific Support Team,
    Thanks you for your reply. However, there are two issues:
    1. Following the link you provided for product no. ab95190 led me to a
    message saying that the product is no longer available in Abcam's
    product range. Do you have any recommendation as to an alternative for
    the S100 standard protein, for use with ab8330?
    2. It looks like for my application, I will need to amplify my sELISA
    signal using a biotinylated system. I'm considering other antibodies
    in your product range which have not been certified by Abcam for
    sELISA, to use as detection antibody. Are you able to provide a small
    amount of sample antibody for testing, say product no. ab48653 or
    ab105371? I intend to go with ab8330 as capture antibody.
    Many thanks for your help again.
    Best wishes,

    Read More
    Answer

    Thank you for your reply.

    My sincere apologies for the confusion. In this case, I am sorry we do not have a suitable standard protein to suggest on this occasion.

    In answer to your second question regarding ab48653 and ab105371, I am sorry these have not been tested in sandwich ELISA and regrettably would not be covered by the guarantee for this application. They have been tested only in indirect ELSA for each antibody against its antigen.

    I am sorry to confirm that because we carry over 90 000 products, it is not possible for use tostock sample vials to send to customers. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact me again by replying to this email prior to purchase if you would like more information.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contactme again with any other questions

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    Question

    Does this antibody recognize S100A or S100B?

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    Answer

    Thank you for your phone call last week. I have heard back from my contact at the lab, and I was told that ab48653 recognizes both the A and B forms of S100.  Please let me know if you have further questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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