Anti-S100 antibody [D28-E] (ab136629)
Key features and details
- Rabbit monoclonal [D28-E] to S100
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-S100 antibody [D28-E]
See all S100 primary antibodies -
Description
Rabbit monoclonal [D28-E] to S100 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human S100 (C terminal).
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Epitope
Antibody recognizes the epitope located between Val55 - Glu69 -
Positive control
- Human neurofibroma, skin melanoma, cutaneous malignant melanoma, and breast tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.00
Preservative: 0.05% Sodium azide
Constituents: 0.32% Tris HCl, 2% BSA -
Concentration information loading...
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Purification notes
This immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. This antibody is purified using a propriety technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability. -
Clonality
Monoclonal -
Clone number
D28-E -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab136629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/200.
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Notes |
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IHC-P
1/100 - 1/200. |
Target
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Function
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. -
Tissue specificity
Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. -
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 6271 Human
- Omim: 176940 Human
- SwissProt: P23297 Human
- Unigene: 515715 Human
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Alternative names
- Bpb antibody
- NEF antibody
- Protein S100-A1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [D28-E] (ab136629)Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human neurofibroma tissue labelling S100 with ab136629 antibody at 1/100 diltution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [D28-E] (ab136629)Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human skin melanoma tissue labelling S100 with ab136629 antibody at 1/100 diltution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [D28-E] (ab136629)Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human breast tissue labelling S100 with ab136629 antibody at 1/100 diltution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [D28-E] (ab136629)
Immunohistochemical analysis of formalin fixed, paraffin embedded human cutaneous malignant melanoma tissue labeling S100 with ab136629.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab136629 has not yet been referenced specifically in any publications.