Anti-S100 beta antibody [4C4.9] - BSA and Azide free (ab212816)
Key features and details
- Mouse monoclonal [4C4.9] to S100 beta - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
-
Product name
Anti-S100 beta antibody [4C4.9] - BSA and Azide free
See all S100 beta primary antibodies -
Description
Mouse monoclonal [4C4.9] to S100 beta - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan -
Immunogen
Full length native protein (purified) corresponding to Cow S100 beta. Purified bovine brain protein.
Database link: P04271 -
Positive control
- Human melanoma and brain tissues; Human cerebellum lysate; A2058 cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Purification notes
ab212816 was purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
4C4.9 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
(Primary incubation for 30 minutes at RT). |
|
WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 11 kDa.
|
Notes |
---|
IHC-P
Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (Primary incubation for 30 minutes at RT). |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 11 kDa. |
Target
-
Function
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. -
Tissue specificity
Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. -
Sequence similarities
Belongs to the S-101 family.
Contains 2 EF-hand domains. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 6285 Human
- Omim: 176990 Human
- SwissProt: P04271 Human
- Unigene: 422181 Human
-
Alternative names
- NEF antibody
- Protein S100 B antibody
- Protein S100-B antibody
see all
Images
Protocols
Datasheets and documents
-
Datasheet download
References (2)
ab212816 has been referenced in 2 publications.
- Shen M et al. Isolation of rat Schwann cells based on cell sorting. Mol Med Rep 16:1747-1752 (2017). PubMed: 28627700
- Szmydynger-Chodobska J et al. The Involvement of Pial Microvessels in Leukocyte Invasion after Mild Traumatic Brain Injury. PLoS One 11:e0167677 (2016). PubMed: 28030563