Anti-S100 beta antibody (ab41548)
Key features and details
- Rabbit polyclonal to S100 beta
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Zebrafish
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-S100 beta antibody
See all S100 beta primary antibodies -
Description
Rabbit polyclonal to S100 beta -
Host species
Rabbit -
Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab52642.
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Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Zebrafish
Predicted to work with: Rabbit, Cow, Human, Pig, Chinese hamster -
Immunogen
Synthetic peptide corresponding to Rat S100 beta aa 50 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab41547) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (10) |
Use at an assay dependent concentration.
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 11 kDa).
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Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 11 kDa). |
Target
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Function
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. -
Tissue specificity
Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. -
Sequence similarities
Belongs to the S-101 family.
Contains 2 EF-hand domains. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 525716 Cow
- Entrez Gene: 6285 Human
- Entrez Gene: 20203 Mouse
- Entrez Gene: 100009495 Rabbit
- Entrez Gene: 25742 Rat
- Omim: 176990 Human
- SwissProt: P02638 Cow
- SwissProt: P04271 Human
see all -
Alternative names
- NEF antibody
- Protein S100 B antibody
- Protein S100-B antibody
see all
Images
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ab41548 staining S100 beta in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/10000 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
At this dilution factor, there is very good delineation of astrocyte processes.
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All lanes : Anti-S100 beta antibody (ab41548) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Spinal Cord (Mouse) Tissue Lysate
Lane 4 : Spinal Cord (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 11 kDa
Observed band size: 9 kDa why is the actual band size different from the predicted?
Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands. -
ab41548 staining S100 beta in Zebrafish body, showing T/S of spinal cord region by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/10000 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (98)
ab41548 has been referenced in 98 publications.
- Raus AM et al. Early-life exercise primes the murine neural epigenome to facilitate gene expression and hippocampal memory consolidation. Commun Biol 6:18 (2023). PubMed: 36611093
- Gangwani MR et al. Neuronal and astrocytic contributions to Huntington's disease dissected with zinc finger protein transcriptional repressors. Cell Rep 42:111953 (2023). PubMed: 36640336
- Senko AN et al. Systems genetics in the rat HXB/BXH family identifies Tti2 as a pleiotropic quantitative trait gene for adult hippocampal neurogenesis and serum glucose. PLoS Genet 18:e1009638 (2022). PubMed: 35377872
- Brezovakova V et al. Astrocytes Derived from Familial and Sporadic Alzheimer's Disease iPSCs Show Altered Calcium Signaling and Respond Differently to Misfolded Protein Tau. Cells 11:N/A (2022). PubMed: 35563735
- Zhao W et al. Astrocytic Nrf2 expression protects spinal cord from oxidative stress following spinal cord injury in a male mouse model. J Neuroinflammation 19:134 (2022). PubMed: 35668451