Mouse, Rat, Goat, Human, Zebrafish, Macaque monkey
Synthetic peptide within Human S100 beta aa 50 to the C-terminus (C terminal). The exact sequence is proprietary. Database link: P04271
IHC-P: Human, mouse and rat cerebral cortex. Human spiral ganglion and melanoma tissue.
WB: B16F0 and A-375 cell lysates, mouse spinal cord, rat brain.
ICC/IF: A-375 cell lysates.
IP: Human fetal brain.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.
Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
Belongs to the S-101 family. Contains 2 EF-hand domains.
S100 calcium binding protein beta (neural) antibody
S100 calcium-binding protein B antibody
S100 protein beta chain antibody
Western blot - Anti-S100 beta antibody [EP1576Y] (ab52642)
Anti-S100 beta antibody [EP1576Y] (ab52642) at 1/5000 dilution (purified) + A-375 (human malignant melanoma cell line) at 10 µg
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 11 kDa Observed band size: 11 kDa
Blocking and Diluting buffer and concentration: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] (ab52642)This image is courtesy of an Abreview submitted by Heiko Locher
Unpurified ab52642 staining S100 beta in human spiral ganglion tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/200 in PBS-T + 1% BSA) for 12 hours. An Alexa Fluor® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 A-375 (human malignant melanoma cell line) cells labeling S100 beta with purified ab52642 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) ab150077 secondary antibody at 1/500 dilution (green). The nuclear counter stain is DAPI (blue). The negative control is as follows; ab52642 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
Immunohistochemical analysis of paraffin embedded rat cerebral cortex tissue labeling S100 beta with purified ab52642 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stain: Hematoxylin.
Negative control: Using PBS instead of primary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-S100 beta antibody [EP1576Y] (ab52642)This image is courtesy of an anonymous abreview.
Immunocytochemistry/ Immunofluorescence analysis of mouse colon-derived neurospheres labeling S100 beta with ab52642 at 1/400 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton X-100. Next the cells were blocked with 5 % serum for 1 hour at 25°C, followed by incubation with anti-S100 beta antibody [EP1576Y] (ab52642) in 1% BSA for 18 hours at 4°C. A polyclonal goat anti-rabbit IgG Alexa Fluor® 594 was used at 1/200 dilution.
S100 beta was immunoprecipitated from human fetal brain with purified ab52642 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab52642 and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as secondary antibody at 1/1000 dilution.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] (ab52642)Image from Selvadurai HJ & Mason JO, PLoS One. 2011;6(8):e23012. Epub 2011 Aug 8. Fig 5.; doi:10.1371/journal.pone.0023012; August 8, 2011, PLoS ONE 6(8): e23012.
Immunohistochemical analysis of embryonic mouse brain tissue, staining S100 beta with unpurified ab52642.
Other - Anti-S100 beta antibody [EP1576Y] (ab52642)
Equilibrium disassociation constant (KD) Learn more about KD
Gerli MFM et al. Perfusion decellularization of a human limb: A novel platform for composite tissue engineering and reconstructive surgery. PLoS One13:e0191497 (2018).
Read more (PubMed: 29352303) »
Brockett AT et al. Evidence supporting a role for astrocytes in the regulation of cognitive flexibility and neuronal oscillations through the Ca2+ binding protein S100ß. PLoS One13:e0195726 (2018).
Read more (PubMed: 29664924) »