Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 beta antibody [EP1576Y]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Adipose)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH 6
Permeabilization
No
Specification
Adipose
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% buffered Formalin

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500

Secondary antibody

Dilution
1/400
Name
Non-Abcam antibody was used: Biotin conjugated Donkey anti-Rabbit secondary ant
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
Elite Vectastain ABC kit (PK-6100) was used.

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Submitted Aug 20 2015

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