Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 beta antibody [EP1576Y]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (large intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Diva Decloaker
Permeabilization
Yes - 0.1% Triton X-100
Specification
large intestine
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA
Dilution
1/100

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594

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Submitted May 16 2016

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