Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 beta antibody [EP1576Y]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Whole Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - Tween-20
Specification
Whole Brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Block
Dilution
1/200

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Alexfluor488 anti Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 488

Additional data

Additional Notes
Tissue was paraffin-embedded, sectioned onto slides at 15 microns. Produced a very bright cellular stain with low background.

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Submitted Mar 29 2017

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