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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Whole Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Yes - Tween-20
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Block
Non-Abcam antibody was used: Alexfluor488 anti Rabbit
Host species: Donkey
Conjugation: Alexa Fluor 488
Tissue was paraffin-embedded, sectioned onto slides at 15 microns.
Produced a very bright cellular stain with low background.
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Submitted Mar 29 2017