Western blot abreview for Anti-S100 beta antibody [EP1576Y]

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole Brain Tissue Lysate)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (3-8% Tris-Acetate)
Loading amount
20 µg
Specification
Whole Brain Tissue Lysate
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Block
Dilution
1/5000

Secondary antibody

Dilution
1/15000
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (H+L) to HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Negative control
No negative control.
Exposure
45 second(s)
Bands
Specific: 11 kDa Non-specific: 25, 50 kDa
Positive control
Lysate from mouse cortex: observed 11, 25 kDa bands.

Additional data

Additional Notes
Used PVDF membrane.

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Submitted Jun 22 2017

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