Immunocytochemistry/ Immunofluorescence abreview for Anti-S100 beta antibody [EP1576Y]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (Spermatogonial stem cells differentiating into gli)
Permeabilization
Yes - 0.1 % Triton-X
Specification
Spermatogonial stem cells differentiating into gli
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1%BSA in PBS with 0.1% Tween20
Dilution
1/100

Secondary antibody

Dilution
1/500

Additional data

Additional Notes
Primary spermatogonial stem cells differentiating into Schwann cells. Laminin substrate, 10 nM human NRG1 Type III peptide, 20% FBS, 4mM glutamate, Neurobasal media.

Dominic Schomberg

Verified customer

Submitted Apr 18 2018

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