Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1576Y] to S100 beta - Astrocyte Marker (Alexa Fluor® 647)
- Suitable for: ICC/IF
- Reacts with: Mouse, Rat, Goat, Human, Zebrafish, Macaque monkey
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Product nameAnti-S100 beta antibody [EP1576Y] - Astrocyte Marker (Alexa Fluor® 647)
See all S100 beta primary antibodies
DescriptionRabbit monoclonal [EP1576Y] to S100 beta - Astrocyte Marker (Alexa Fluor® 647)
ConjugationAlexa Fluor® 647. Ex: 652nm, Em: 668nm
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Goat, Human, Zebrafish, Macaque monkey
Synthetic peptide within Human S100 beta (C terminal). The exact sequence is proprietary.
- ICC/IF: A375 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Dissociation constant (KD)KD = 5.50 x 10 -10 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (HRP) (ab195535)
- Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (Alexa Fluor® 488) (ab196442)
- Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (Alexa Fluor® 568) (ab201837)
- Anti-S100 beta antibody [EP1576Y] - BSA and Azide free (ab215989)
- Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642)
Our Abpromise guarantee covers the use of ab196175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product gave a positive signal in A375 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).
FunctionWeakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.
Tissue specificityAlthough predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
Sequence similaritiesBelongs to the S-101 family.
Contains 2 EF-hand domains.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- NEF antibody
- Protein S100 B antibody
- Protein S100-B antibody
ab196175 staining S100 beta in A375 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196175 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in A375 cells fixed with 4% formaldehyde (10 min).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab196175 has not yet been referenced specifically in any publications.