Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1576Y] to S100 beta - Astrocyte Marker (HRP)
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: HRP
Product nameAnti-S100 beta antibody [EP1576Y] - Astrocyte Marker (HRP)
See all S100 beta primary antibodies
DescriptionRabbit monoclonal [EP1576Y] to S100 beta - Astrocyte Marker (HRP)
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human S100 beta (C terminal). The exact sequence is proprietary.
- IHC-P: Human cerebellum tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Dissociation constant (KD)KD = 5.50 x 10 -10 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (Alexa Fluor® 647) (ab196175)
- Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (Alexa Fluor® 488) (ab196442)
- Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (Alexa Fluor® 568) (ab201837)
- Anti-S100 beta antibody [EP1576Y] - BSA and Azide free (ab215989)
- Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642)
Our Abpromise guarantee covers the use of ab195535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionWeakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.
Tissue specificityAlthough predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
Sequence similaritiesBelongs to the S-101 family.
Contains 2 EF-hand domains.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- NEF antibody
- Protein S100 B antibody
- Protein S100-B antibody
IHC image of S100 beta staining in a section of formalin-fixed paraffin-embedded normal human cerebellum tissue, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195535 at 1/500 dilution, for 15 mins at room temperature.
DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab195535 has not yet been referenced specifically in any publications.