Recombinant Anti-S100 beta antibody [EP1576Y] - BSA and Azide free (ab215989)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1576Y] to S100 beta - BSA and Azide free
- Suitable for: WB, IP, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-S100 beta antibody [EP1576Y] - BSA and Azide free
See all S100 beta primary antibodies -
Description
Rabbit monoclonal [EP1576Y] to S100 beta - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Goat, Zebrafish, Macaque monkey -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human, mouse and rat cerebral cortex. Human spiral ganglion and melanoma tissue; Normal WT and laser-treated mouse retina; Native and acellular peripheral nerve sections; Embryonic mouse brain tissue, brain tissue; WB: B16F0 and A-375 cell lysates, mouse spinal cord, rat brain; ICC/IF: A-375 cells. IP: Human fetal brain; IHC-Fr: Mouse and rat cerebrum, Hu cerebral cortex
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General notes
ab215989 is the carrier-free version of ab52642.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 5.50 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1576Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- HRP Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab195535)
- Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab196175)
- Alexa Fluor® 488 Anti-S100 beta antibody [EP1576Y] (ab310990)
- Alexa Fluor® 568 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab313015)
- Alexa Fluor® 555 Anti-S100 beta antibody [EP1576Y] (ab313219)
- Alexa Fluor® 568 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab313817)
- Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215989 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
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IP |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IHC-Fr | (1) |
Use at an assay dependent concentration.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
Use at an assay dependent concentration. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. -
Tissue specificity
Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. -
Sequence similarities
Belongs to the S-101 family.
Contains 2 EF-hand domains. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6285 Human
- Entrez Gene: 20203 Mouse
- Entrez Gene: 25742 Rat
- Omim: 176990 Human
- SwissProt: P04271 Human
- SwissProt: P50114 Mouse
- SwissProt: P04631 Rat
- Unigene: 422181 Human
see all -
Alternative names
- NEF antibody
- Protein S100 B antibody
- Protein S100-B antibody
see all
Images
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This data was developed using the same antibody clone in a different buffer formulation (ab52642).
IHC image of S100 beta staining in a section of frozen normal human cerebral cortex performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab16659, 1/5000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 A-375 (human malignant melanoma cell line) cells labeling S100 beta with purified ab52642 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) ab150077 secondary antibody at 1/500 dilution (green). The nuclear counter stain is DAPI (blue). The negative control is as follows;
ab52642 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52642).
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Immunohistochemical analysis of paraffin embedded human cerebral cortex tissue labeling S100 beta with purified ab52642 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stain: Hematoxylin.
Negative control: Using PBS instead of primary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52642).
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Immunohistochemistry (Frozen sections) analysis of rat cerebrum tissue sections labeling S100 beta with Purified ab52642 at 1/100 (9.9 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52642).
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Immunohistochemistry (Frozen sections) analysis of mouse cerebrum tissue sections labeling S100 beta with Purified ab52642 at 1/100 (9.9 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52642).
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Immunohistochemical analysis of paraffin embedded rat cerebral cortex tissue labeling S100 beta with purified ab52642 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stain: Hematoxylin.
Negative control: Using PBS instead of primary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52642).
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This data was developed using ab52642, the same antibody clone in a different buffer formulation.
S100 beta antibody ab52642 was used with Tissue Clearing Kit ab243298 to penetrate, stain and clear a 1 mm coronal section of mouse brain. Blue: DAPI, Green: S100 beta.
Learn more about tissue clearing kits, reagents, and protocols designed to make it easier to stain thick tissue sections and get more data from each valuable tissue section.
To use this antibody with tissue clearing, use Tissue Clearing Kit ab243298. For 1 mm brain sections, we recommend a starting dilution of 1:200, and also using Goat Anti-Rabbit IgG H&L AlexaFluor488 (ab150077) at a dilution of 1:400.
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Immunohistochemical analysis of paraffin embedded mouse cerebral cortex tissue labeling S100 beta with purified ab52642 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stain: Hematoxylin.
Negative control: Using PBS instead of primary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52642).
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S100 beta is present in CNV lesions.
A) S100 beta expression in a normal WT mouse retina. Strong immunoreactivity is present in the astrocytes (arrow). The position of the inner nuclear layer (INL) and outer nuclear layer (ONL) are indicted. Scale bar is 50 µm B) S100 beta expression in WT mouse retinal at day 7 post-laser treatment. S100B was detected in the outer plexiform layer (arrowheads). Strong S100 beta expression was detected at the site of CNV. Scale bar is 50 µm
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52642).
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Immunofluorescent imaging of human native and acellular peripheral nerve sections stained for the axon protein Neurofilaments (NF200), the Schwann’s cell marker S100β (ab52642) and for the extracellular matrix proteins Laminin and Collagen IV. Sections were counterstained with DAPI to confirm the removal of the cell nuclei upon decellularization (scale bar: 100 μm).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52642).
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Unpurified ab52642 staining S100 beta in human spiral ganglion tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/200 in PBS-T + 1% BSA) for 12 hours. An Alexa Fluor® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52642).
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Clone EP1576Y (ab215989) has been successfully conjugated by Abcam. This image was generated using Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (Alexa Fluor® 488). Please refer to ab196442 for protocol details.
ab196442 staining S100 beta in A375 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196442 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in A375 cells fixed with 4% formaldehyde (10 min).
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Clone EP1576Y (ab215989) has been successfully conjugated by Abcam. This image was generated using Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (Alexa Fluor® 647). Please refer to ab196175 for protocol details.
ab196175 staining S100 beta in A375 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196175 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in A375 cells fixed with 4% formaldehyde (10 min).
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Immunocytochemistry/ Immunofluorescence analysis of mouse colon-derived neurospheres labeling S100 beta with ab52642 at 1/400 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton X-100. Next the cells were blocked with 5 % serum for 1 hour at 25°C, followed by incubation with anti-S100 beta antibody [EP1576Y] (ab52642) in 1% BSA for 18 hours at 4°C. A polyclonal goat anti-rabbit IgG Alexa Fluor® 594 was used at 1/200 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52642).
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S100 beta was immunoprecipitated from human fetal brain with purified ab52642 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab52642 and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as secondary antibody at 1/1000 dilution.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52642).
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Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52642).
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab215989 has not yet been referenced specifically in any publications.