Recombinant
RabMAb

Recombinant Anti-S100 beta antibody [EP1576Y] (HRP) (ab195535)

Overview

  • Product name
    Anti-S100 beta antibody [EP1576Y] (HRP)
    See all S100 beta primary antibodies
  • Description
    Rabbit monoclonal [EP1576Y] to S100 beta (HRP)
  • Host species
    Rabbit
  • Conjugation
    HRP
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human S100 beta (C terminal). The exact sequence is proprietary.

  • Positive control
    • IHC: Human cerebellum tissue
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab195535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.
  • Tissue specificity
    Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
  • Sequence similarities
    Belongs to the S-101 family.
    Contains 2 EF-hand domains.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • NEF antibody
    • Protein S100 B antibody
    • Protein S100-B antibody
    • S 100 calcium binding protein beta chain antibody
    • S 100 protein beta chain antibody
    • S-100 protein beta chain antibody
    • S-100 protein subunit beta antibody
    • S100 antibody
    • S100 calcium binding protein beta (neural) antibody
    • S100 calcium-binding protein B antibody
    • S100 protein beta chain antibody
    • S100B antibody
    • S100B_HUMAN antibody
    • S100beta antibody
    see all

Images

  • IHC image of S100 beta staining in a section of formalin-fixed paraffin-embedded normal human cerebellum tissue, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195535 at 1/500 dilution, for 15 mins at room temperature.

    DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

ab195535 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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