Recombinant Anti-S100 beta antibody [SP127] (ab115803)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] (ab115803)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP127] to S100 beta
Suitable for: IHC-P, Flow Cyt
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-S100 beta antibody [SP127]
See all S100 beta primary antibodies
Description

Rabbit monoclonal [SP127] to S100 beta
Host species

Rabbit
Tested applications

Suitable for: IHC-P, Flow Cytmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow
Immunogen

Recombinant full length protein corresponding to Human S100 beta.
Database link: P04271
Positive control
- IHC-P: Human colon, melanoma, kidney, colon, spinal cord, cerebellum, adrenal gland bladder, breast, oesophagus, Mouse cerebrum, and Rat cerebrum tissue; Flow Cyt: A375 cells.
General notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by Protein A/G.
Clonality

Monoclonal
Clone number

SP127
Isotype

IgG
Research areas
- Neuroscience
- Development

Alternative Versions
- Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human S100 beta protein (ab55570)
Related Products
- Recombinant human S100 beta protein (ab200489)
- Recombinant Human S100 beta protein (ab55570)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab115803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/100. Incubate with primary antibody for 10 minutes at room temperature.
Flow Cyt		Use at an assay dependent concentration.

Notes
IHC-P 1/100. Incubate with primary antibody for 10 minutes at room temperature.
Flow Cyt Use at an assay dependent concentration.

Function

Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.
Tissue specificity

Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
Sequence similarities

Belongs to the S-101 family.
Contains 2 EF-hand domains.
Cellular localization

Cytoplasm. Nucleus.
Target information above from: UniProt accession P04271 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 525716 Cow
- Entrez Gene: 6285 Human
- Entrez Gene: 20203 Mouse
- Entrez Gene: 100009495 Rabbit
- Entrez Gene: 25742 Rat
- Omim: 176990 Human
- SwissProt: P02638 Cow
- SwissProt: P04271 Human
- SwissProt: P50114 Mouse
- SwissProt: Q6YNR6 Rabbit
- SwissProt: P04631 Rat
- Unigene: 422181 Human
- Unigene: 235998 Mouse
- Unigene: 8937 Rat
see all
Alternative names
- NEF antibody
- Protein S100 B antibody
- Protein S100-B antibody
- S 100 calcium binding protein beta chain antibody
- S 100 protein beta chain antibody
- S-100 protein beta chain antibody
- S-100 protein subunit beta antibody
- S100 antibody
- S100 calcium binding protein beta (neural) antibody
- S100 calcium-binding protein B antibody
- S100 protein beta chain antibody
- S100B antibody
- S100B_HUMAN antibody
- S100beta antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] (ab115803)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue sections labeling S100 beta with ab115803 at 1/100 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] (ab115803)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling S100 beta with ab115803 at 1/100 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] (ab115803)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast tissue sections labeling S100 beta with ab115803 at 1/100 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] (ab115803)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling S100 beta with ab115803 at 1/100 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] (ab115803)

Formalin-fixed, paraffin-embedded human cerebellum tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.
Flow Cytometry - Anti-S100 beta antibody [SP127] (ab115803)

Flow Cytometry analysis of A375(Human malignant melanoma epithelial cell) cells labeling S100 beta with purified ab115803 Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] (ab115803)

Formalin-fixed, paraffin-embedded human spinal cord tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] (ab115803)

Formalin-fixed, paraffin-embedded human melanoma tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] (ab115803)

Formalin-fixed, paraffin-embedded human melanoma tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] (ab115803)

Formalin-fixed, paraffin-embedded human esophagus tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] (ab115803)

Formalin-fixed, paraffin-embedded human breast tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] (ab115803)

Formalin-fixed, paraffin-embedded human bladder tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] (ab115803)

Formalin-fixed, paraffin-embedded human adrenal gland tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] (ab115803)

Formalin-fixed, paraffin-embedded human colon tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] (ab115803)

Formalin-fixed, paraffin-embedded human kidney tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] (ab115803)

Formalin-fixed, paraffin-embedded human colon tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.
Anti-S100 beta antibody [SP127] (ab115803)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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Datasheet download

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Publishing research using ab115803? Please let us know so that we can cite the reference in this datasheet.

ab115803 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Purification notes

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (0)

Customer reviews and Q&As