Recombinant Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
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Overview
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Product name
Anti-S100A10 antibody [EPR3317] - BSA and Azide free
See all S100A10 primary antibodies -
Description
Rabbit monoclonal [EPR3317] to S100A10 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human S100A10 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P60903 -
Positive control
- IHC-P: Human squamous cell cervical carcinoma tissue.
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General notes
ab232524 is the carrier-free version of ab76472 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab232524 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
The mouse recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse samples.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3317 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab232524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 11 kDa. |
Target
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Function
Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase. -
Sequence similarities
Belongs to the S-100 family. - Information by UniProt
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Database links
- Entrez Gene: 6281 Human
- Entrez Gene: 20194 Mouse
- Omim: 114085 Human
- SwissProt: P60903 Human
- SwissProt: P08207 Mouse
- Unigene: 143873 Human
- Unigene: 1 Mouse
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Alternative names
- 42C antibody
- AA409961 antibody
- AL024248 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)](https://www.abcam.com/ps/products/232/ab232524/Images/ab232524-3-ab764722.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)Unpurified ab76472 at 1/100 dilution staining S100A10 in human colon tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76472).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)](https://www.abcam.com/ps/products/232/ab232524/Images/ab232524-2-ab764724.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)Unpurified ab76472 at 1/100 dilution staining S100A10 in human kidney tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76472).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)](https://www.abcam.com/ps/products/232/ab232524/Images/ab232524-308853-232524.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)Immunohistochemical staining of paraffin embedded human squamous cell cervical carcinoma with purified ab76472 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76472).
Datasheets and documents
References
ab232524 has not yet been referenced specifically in any publications.
