For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    s100a10-antibody-epr3317-bsa-and-azide-free-ab232524.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Cytoskeleton / ECM Cell Adhesion Annexins
Share by email
RecombinantRabMAb

Recombinant Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
  • Western blot - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
  • Western blot - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
  • Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3317] to S100A10 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human

You may also be interested in

Conjugation
Product image
Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)
Knockout
Product image
Human S100A10 knockout HEK293T cell lysate (ab257649)
Protein
Product image
Recombinant Human S100A10 protein (ab55671)

View more associated products

Overview

  • Product name

    Anti-S100A10 antibody [EPR3317] - BSA and Azide free
    See all S100A10 primary antibodies
  • Description

    Rabbit monoclonal [EPR3317] to S100A10 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human S100A10 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P60903

  • Positive control

    • WB: HaCaT, A431, SW480, RAW264.7, NIH/3T3, and HT-29 cell lysates; IHC-P: Human squamous cell cervical carcinoma, colon tissue and human kidney tissue.
  • General notes

    ab232524 is the carrier-free version of ab76472 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab232524 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    The mouse recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse samples.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3317
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Annexins
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins

Associated products

  • Alternative Versions

    • Anti-S100A10 antibody [EPR3317] (ab76472)
  • Conjugation kits

    • FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human S100A10 protein (ab55671)

Applications

Our Abpromise guarantee covers the use of ab232524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.
    • Sequence similarities

      Belongs to the S-100 family.
    • Target information above from: UniProt accession P60903 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6281 Human
      • Entrez Gene: 20194 Mouse
      • Omim: 114085 Human
      • SwissProt: P60903 Human
      • SwissProt: P08207 Mouse
      • Unigene: 143873 Human
      • Unigene: 1 Mouse
      • Alternative names

        • 42C antibody
        • AA409961 antibody
        • AL024248 antibody
        • Annexin II ligand antibody
        • Annexin II ligand, calpactin I, light polypeptide antibody
        • Annexin II tetramer (AIIt) p11 subunit antibody
        • Annexin II, light chain antibody
        • ANX2L antibody
        • ANX2LG antibody
        • Ca[1] antibody
        • CAL12 antibody
        • CAL1L antibody
        • Calpactin I light chain antibody
        • Calpactin I, p11 subunit antibody
        • Calpactin-1 light chain antibody
        • Cellular ligand of annexin II antibody
        • CLP11 antibody
        • GP11 antibody
        • MGC111133 antibody
        • Nerve growth factor-induced protein 42C antibody
        • OTTHUMP00000015269 antibody
        • OTTHUMP00000015270 antibody
        • p10 antibody
        • p10 protein antibody
        • p11 antibody
        • Protein S100 A10 antibody
        • Protein S100-A10 antibody
        • S100 calcium binding protein A10 (annexin II ligand, calpactin I, light polypeptide (p11)) antibody
        • S100 calcium binding protein A10 (calpactin) antibody
        • S100 calcium binding protein A10 antibody
        • S100 calcium-binding protein A10 antibody
        • S100a10 antibody
        • S10AA_HUMAN antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)

        This data was developed using ab76472, the same antibody clone in a different buffer formulation.

        Immunohistochemical staining of paraffin embedded human squamous cell cervical carcinoma with purified ab76472 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

      • Western blot - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
        Western blot - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
        All lanes : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/10000 dilution (unpurified)

        Lane 1 : HaCaT (Human skin keratinocyte) whole cell lysate
        Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
        Lane 3 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
        Lane 4 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 11 kDa
        Observed band size: 13 kDa
        why is the actual band size different from the predicted?
        Additional bands at: 40 kDa (possible non-specific binding)


        Exposure time: 180 seconds


        Blocking buffer: 5% NFDM/TBST

        This data was developed using ab76472, the same antibody clone in a different buffer formulation.

      • Western blot - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
        Western blot - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
        Lane 1 : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/1000 dilution (purified)
        Lane 2 : Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) at 1/5000 dilution

        All lanes : Recombinant Human S100A10 protein (His tag) (ab221399)

        Lysates/proteins at 0.01 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 11 kDa
        Observed band size: 13 kDa why is the actual band size different from the predicted?
        Additional bands at: 25 kDa (possible dimer)


        Exposure time: 3 seconds


        Blocking buffer: 5% NFDM/TBST

        This data was developed using ab76472, the same antibody clone in a different buffer formulation.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)

        This data was developed using ab76472, the same antibody clone in a different buffer formulation.

        Unpurified ab76472 at 1/100 dilution staining S100A10 in human colon tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)

        This data was developed using ab76472, the same antibody clone in a different buffer formulation.

        Unpurified ab76472 at 1/100 dilution staining S100A10 in human kidney tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
        Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab232524? Please let us know so that we can cite the reference in this datasheet.

      ab232524 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab232524.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.