Product nameAnti-S100A11 antibody [EPR11171(B)]
See all S100A11 primary antibodies
DescriptionRabbit monoclonal [EPR11171(B)] to S100A11
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues in Human S100A11 (UniProt P31949).
- SKBR3, BxPC-3, PC-3 and HACAT cell lysates; BxPC-3 cells; HACAT cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab169530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 11 kDa.|
|ICC/IF||1/250 - 1/500.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionFacilitates the differentiation and the cornification of keratinocytes.
Sequence similaritiesBelongs to the S-100 family.
Contains 2 EF-hand domains.
modificationsPhosphorylation at Thr-10 by PRKCA significantly suppresses homodimerization and promotes association with NCL/nucleolin which induces nuclear translocation.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Calgizzarin antibody
- Epididymis secretory protein Li 43 antibody
- HEL S 43 antibody
All lanes : Anti-S100A11 antibody [EPR11171(B)] (ab169530) at 1/1000 dilution
Lane 1 : SKBR3 cell lysate
Lane 2 : BxPC-3 cell lysate
Lane 3 : PC-3 cell lysate
Lane 4 : HACAT cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 11 kDa
Immunofluorescent analysis of BxPC-3 cells labeling S100A11 with ab169530 at 1/250 dilution. DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized HACAT cells labeling S100A11 with ab169530 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
ab169530 has not yet been referenced specifically in any publications.