Anti-S100A11 antibody [EPR11171(B)] (ab169530)
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Overview
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Product nameAnti-S100A11 antibody [EPR11171(B)]
See all S100A11 primary antibodies -
DescriptionRabbit monoclonal [EPR11171(B)] to S100A11
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide corresponding to residues in Human S100A11 (UniProt P31949).
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Positive control
- SKBR3, BxPC-3, PC-3 and HACAT cell lysates; BxPC-3 cells; HACAT cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR11171(B)
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab169530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 11 kDa. | |
| ICC/IF | 1/250 - 1/500. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionFacilitates the differentiation and the cornification of keratinocytes.
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Sequence similaritiesBelongs to the S-100 family.
Contains 2 EF-hand domains. -
Post-translational
modificationsPhosphorylation at Thr-10 by PRKCA significantly suppresses homodimerization and promotes association with NCL/nucleolin which induces nuclear translocation. -
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
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Database links
- Entrez Gene: 6282 Human
- Omim: 603114 Human
- SwissProt: P31949 Human
- Unigene: 417004 Human
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Alternative names
- Calgizzarin antibody
- Epididymis secretory protein Li 43 antibody
- HEL S 43 antibody
see all
Images
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![Western blot - Anti-S100A11 antibody [EPR11171(B)] (ab169530)](https://www.abcam.com/ps/datasheet/images/169/ab169530/S100A11-Primary-antibodies-ab169530-1.jpg)
Western blot - Anti-S100A11 antibody [EPR11171(B)] (ab169530)All lanes : Anti-S100A11 antibody [EPR11171(B)] (ab169530) at 1/1000 dilution
Lane 1 : SKBR3 cell lysate
Lane 2 : BxPC-3 cell lysate
Lane 3 : PC-3 cell lysate
Lane 4 : HACAT cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 11 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11171(B)] (ab169530)](https://www.abcam.com/ps/datasheet/images/169/ab169530/S100A11-Primary-antibodies-ab169530-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11171(B)] (ab169530)Immunofluorescent analysis of BxPC-3 cells labeling S100A11 with ab169530 at 1/250 dilution. DAPI nuclear staining (blue). -
![Flow Cytometry - Anti-S100A11 antibody [EPR11171(B)] (ab169530)](https://www.abcam.com/ps/datasheet/images/169/ab169530/S100A11-Primary-antibodies-ab169530-3.jpg)
Flow Cytometry - Anti-S100A11 antibody [EPR11171(B)] (ab169530)Flow cytometric analysis of permeabilized HACAT cells labeling S100A11 with ab169530 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
Protocols
Datasheets and documents
References
ab169530 has not yet been referenced specifically in any publications.
