Recombinant
RabMAb

Recombinant Anti-S100A11 antibody [EPR11171(B)] (ab169530)

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Overview

  • Product name

    Anti-S100A11 antibody [EPR11171(B)]
    See all S100A11 primary antibodies

  • Description

    Rabbit monoclonal [EPR11171(B)] to S100A11

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to Human S100A11.

  • Positive control

    • SKBR3, BxPC-3, PC-3 and HACAT cell lysates; BxPC-3 cells; HACAT cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab169530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 11 kDa.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Facilitates the differentiation and the cornification of keratinocytes.

    • Sequence similarities

      Belongs to the S-100 family.
      Contains 2 EF-hand domains.

    • Post-translational
      modifications

      Phosphorylation at Thr-10 by PRKCA significantly suppresses homodimerization and promotes association with NCL/nucleolin which induces nuclear translocation.

    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • Calgizzarin antibody
        • Epididymis secretory protein Li 43 antibody
        • HEL S 43 antibody
        • Metastatic lymph node gene 70 protein antibody
        • MLN 70 antibody
        • MLN70 antibody
        • Protein S100 A11 antibody
        • Protein S100-A11 antibody
        • Protein S100-A11, N-terminally processed antibody
        • Protein S100-C antibody
        • Protein S100A11 antibody
        • Protein S100C antibody
        • S100 A11 antibody
        • S100 calcium binding protein A11 antibody
        • S100 calcium-binding protein A11 (calgizzarin) antibody
        • S100 calcium-binding protein A11 antibody
        • S100a11 antibody
        • S100C antibody
        • S10AB_HUMAN antibody
        see all

      Images

      • Western blot - Anti-S100A11 antibody [EPR11171(B)] (ab169530)
        Western blot - Anti-S100A11 antibody [EPR11171(B)] (ab169530)
        All lanes : Anti-S100A11 antibody [EPR11171(B)] (ab169530) at 1/1000 dilution

        Lane 1 : SKBR3 cell lysate
        Lane 2 : BxPC-3 cell lysate
        Lane 3 : PC-3 cell lysate
        Lane 4 : HACAT cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 11 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11171(B)] (ab169530)
        Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11171(B)] (ab169530)
        Immunofluorescent analysis of BxPC-3 cells labeling S100A11 with ab169530 at 1/250 dilution. DAPI nuclear staining (blue).
      • Flow Cytometry - Anti-S100A11 antibody [EPR11171(B)] (ab169530)
        Flow Cytometry - Anti-S100A11 antibody [EPR11171(B)] (ab169530)
        Flow cytometric analysis of permeabilized HACAT cells labeling S100A11 with ab169530 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

      References

      ab169530 has not yet been referenced specifically in any publications.

      Publishing research using ab169530? Please let us know so that we can cite the reference in this datasheet.

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