RecombinantRabMAb

Recombinant Anti-S100A11 antibody [EPR11171(B)] (ab169530)

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Western blot - Anti-S100A11 antibody [EPR11171(B)] (ab169530)
  • Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11171(B)] (ab169530)
  • Flow Cytometry - Anti-S100A11 antibody [EPR11171(B)] (ab169530)
  • Anti-S100A11 antibody [EPR11171(B)] (ab169530)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11171(B)] to S100A11
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-S100A11 antibody [EPR11171(B)]
    See all S100A11 primary antibodies

  • Description

    Rabbit monoclonal [EPR11171(B)] to S100A11

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to Human S100A11.

  • Positive control

    • SKBR3, BxPC-3, PC-3 and HACAT cell lysates; BxPC-3 cells; HACAT cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab169530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 11 kDa.
ICC/IF
1/250 - 1/500.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 11 kDa.
ICC/IF
1/250 - 1/500.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Facilitates the differentiation and the cornification of keratinocytes.

    • Sequence similarities

      Belongs to the S-100 family.
      Contains 2 EF-hand domains.

    • Post-translational
      modifications

      Phosphorylation at Thr-10 by PRKCA significantly suppresses homodimerization and promotes association with NCL/nucleolin which induces nuclear translocation.

    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • Calgizzarin antibody
        • Epididymis secretory protein Li 43 antibody
        • HEL S 43 antibody
        • Metastatic lymph node gene 70 protein antibody
        • MLN 70 antibody
        • MLN70 antibody
        • Protein S100 A11 antibody
        • Protein S100-A11 antibody
        • Protein S100-A11, N-terminally processed antibody
        • Protein S100-C antibody
        • Protein S100A11 antibody
        • Protein S100C antibody
        • S100 A11 antibody
        • S100 calcium binding protein A11 antibody
        • S100 calcium-binding protein A11 (calgizzarin) antibody
        • S100 calcium-binding protein A11 antibody
        • S100a11 antibody
        • S100C antibody
        • S10AB_HUMAN antibody
        see all

      Images

      • Western blot - Anti-S100A11 antibody [EPR11171(B)] (ab169530)
        Western blot - Anti-S100A11 antibody [EPR11171(B)] (ab169530)
        All lanes : Anti-S100A11 antibody [EPR11171(B)] (ab169530) at 1/1000 dilution

        Lane 1 : SKBR3 cell lysate
        Lane 2 : BxPC-3 cell lysate
        Lane 3 : PC-3 cell lysate
        Lane 4 : HACAT cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 11 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11171(B)] (ab169530)
        Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11171(B)] (ab169530)
        Immunofluorescent analysis of BxPC-3 cells labeling S100A11 with ab169530 at 1/250 dilution. DAPI nuclear staining (blue).
      • Flow Cytometry - Anti-S100A11 antibody [EPR11171(B)] (ab169530)
        Flow Cytometry - Anti-S100A11 antibody [EPR11171(B)] (ab169530)
        Flow cytometric analysis of permeabilized HACAT cells labeling S100A11 with ab169530 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
      • Anti-S100A11 antibody [EPR11171(B)] (ab169530)
        Anti-S100A11 antibody [EPR11171(B)] (ab169530)

      References (0)

      Publishing research using ab169530? Please let us know so that we can cite the reference in this datasheet.

      ab169530 has not yet been referenced specifically in any publications.

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