Recombinant Anti-S100A11 antibody [EPR11171(B)] (ab169530)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11171(B)] to S100A11
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-S100A11 antibody [EPR11171(B)]
See all S100A11 primary antibodies -
Description
Rabbit monoclonal [EPR11171(B)] to S100A11 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human S100A11.
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Positive control
- SKBR3, BxPC-3, PC-3 and HACAT cell lysates; BxPC-3 cells; HACAT cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11171(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 11 kDa.
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ICC/IF |
1/250 - 1/500.
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Flow Cyt |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 11 kDa. |
ICC/IF
1/250 - 1/500. |
Flow Cyt
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Facilitates the differentiation and the cornification of keratinocytes. -
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
Post-translational
modificationsPhosphorylation at Thr-10 by PRKCA significantly suppresses homodimerization and promotes association with NCL/nucleolin which induces nuclear translocation. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6282 Human
- Omim: 603114 Human
- SwissProt: P31949 Human
- Unigene: 417004 Human
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Alternative names
- Calgizzarin antibody
- Epididymis secretory protein Li 43 antibody
- HEL S 43 antibody
see all
Images
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All lanes : Anti-S100A11 antibody [EPR11171(B)] (ab169530) at 1/1000 dilution
Lane 1 : SKBR3 cell lysate
Lane 2 : BxPC-3 cell lysate
Lane 3 : PC-3 cell lysate
Lane 4 : HACAT cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 11 kDa -
Immunofluorescent analysis of BxPC-3 cells labeling S100A11 with ab169530 at 1/250 dilution. DAPI nuclear staining (blue).
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Flow cytometric analysis of permeabilized HACAT cells labeling S100A11 with ab169530 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab169530 has not yet been referenced specifically in any publications.