Anti-S100A11 antibody [EPR11171(B)] Recombinant (ab169530)

Product name

Anti-S100A11 antibody [EPR11171(B)]
See all S100A11 primary antibodies
Description

Rabbit monoclonal [EPR11171(B)] to S100A11
Host species

Rabbit
Tested applications

Suitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide corresponding to residues in Human S100A11 (UniProt P31949).
Positive control
- SKBR3, BxPC-3, PC-3 and HACAT cell lysates; BxPC-3 cells; HACAT cells.
General notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11171(B)
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab169530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/5000. Predicted molecular weight: 11 kDa.
ICC/IF		1/250 - 1/500.
Flow Cyt		1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application

Abreviews

Notes

WB

1/1000 - 1/5000. Predicted molecular weight: 11 kDa.

ICC/IF

1/250 - 1/500.

Flow Cyt

1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes

Is unsuitable for IHC-P or IP.

Function

Facilitates the differentiation and the cornification of keratinocytes.
Sequence similarities

Belongs to the S-100 family.
Contains 2 EF-hand domains.
Post-translational
modifications

Phosphorylation at Thr-10 by PRKCA significantly suppresses homodimerization and promotes association with NCL/nucleolin which induces nuclear translocation.
Cellular localization

Cytoplasm. Nucleus.
Target information above from: UniProt accession P31949 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6282 Human
- Omim: 603114 Human
- SwissProt: P31949 Human
- Unigene: 417004 Human
Alternative names
- Calgizzarin antibody
- Epididymis secretory protein Li 43 antibody
- HEL S 43 antibody
- Metastatic lymph node gene 70 protein antibody
- MLN 70 antibody
- MLN70 antibody
- Protein S100 A11 antibody
- Protein S100-A11, N-terminally processed antibody
- Protein S100-C antibody
- Protein S100A11 antibody
- Protein S100C antibody
- S100 A11 antibody
- S100 calcium binding protein A11 antibody
- S100 calcium-binding protein A11 (calgizzarin) antibody
- S100 calcium-binding protein A11 antibody
- S100a11 antibody
- S100C antibody
- S10AB_HUMAN antibody
see all

Western blot - Anti-S100A11 antibody [EPR11171(B)] (ab169530)

All lanes : Anti-S100A11 antibody [EPR11171(B)] (ab169530) at 1/1000 dilution

Lane 1 : SKBR3 cell lysate
Lane 2 : BxPC-3 cell lysate
Lane 3 : PC-3 cell lysate
Lane 4 : HACAT cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 11 kDa
Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11171(B)] (ab169530)

Immunofluorescent analysis of BxPC-3 cells labeling S100A11 with ab169530 at 1/250 dilution. DAPI nuclear staining (blue).
Flow Cytometry - Anti-S100A11 antibody [EPR11171(B)] (ab169530)

Flow cytometric analysis of permeabilized HACAT cells labeling S100A11 with ab169530 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

ab169530 has not yet been referenced specifically in any publications.

Publishing research using ab169530? Please let us know so that we can cite the reference in this datasheet.

Anti-S100A11 antibody [EPR11171(B)] (ab169530)