Recombinant
RabMAb

Recombinant Anti-S100A11 antibody [EPR11172] (Phycoerythrin) (ab211996)

Overview

  • Product name

    Anti-S100A11 antibody [EPR11172] (Phycoerythrin)
    See all S100A11 primary antibodies
  • Description

    Rabbit monoclonal [EPR11172] to S100A11 (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human S100A11 aa 1-100. The exact sequence is proprietary.
    Database link: P31949

  • Positive control

    • Flow Cytometry: PC3 cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab211996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Facilitates the differentiation and the cornification of keratinocytes.
  • Sequence similarities

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Post-translational
    modifications

    Phosphorylation at Thr-10 by PRKCA significantly suppresses homodimerization and promotes association with NCL/nucleolin which induces nuclear translocation.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Calgizzarin antibody
    • Epididymis secretory protein Li 43 antibody
    • HEL S 43 antibody
    • Metastatic lymph node gene 70 protein antibody
    • MLN 70 antibody
    • MLN70 antibody
    • Protein S100 A11 antibody
    • Protein S100-A11 antibody
    • Protein S100-A11, N-terminally processed antibody
    • Protein S100-C antibody
    • Protein S100A11 antibody
    • Protein S100C antibody
    • S100 A11 antibody
    • S100 calcium binding protein A11 antibody
    • S100 calcium-binding protein A11 (calgizzarin) antibody
    • S100 calcium-binding protein A11 antibody
    • S100a11 antibody
    • S100C antibody
    • S10AB_HUMAN antibody
    see all

Images

  • Overlay histogram showing PC3 cells stained with ab211996 (red line). The cells were fixed with 4% formaldehyde and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab211996, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    This antibody gave a positive signal in PC3 cells fixed with 80% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

References

ab211996 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab211996.
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