Key features and details
- Rabbit polyclonal to S100A14
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-S100A14 antibody
See all S100A14 primary antibodies
DescriptionRabbit polyclonal to S100A14
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Mouse, Cow
- IHC-P: Human skin cancer tissue. WB: Recombinant human S100A14; Pig stomach tissue lysate.
This product was previously labelled as S100 calcium binding protein A14
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 12 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionModulates P53/TP53 protein levels, and thereby plays a role in the regulation of cell survival and apoptosis. Depending on the context, it can promote cell proliferation or apoptosis. Plays a role in the regulation of cell migration by modulating the levels of MMP2, a matrix protease that is under transcriptional control of P53/TP53. Does not bind calcium.
Tissue specificityExpressed at highest levels in colon and at moderate levels in thymus, kidney, liver, small intestine, and lung. Low expression in heart and no expression is seen in brain, skeletal muscle, spleen, placenta and peripheral blood leukocytes.
Sequence similaritiesBelongs to the S-100 family.
Contains 1 EF-hand domain.
- Information by UniProt
- BCMP84 antibody
- Protein S100-A14 antibody
- S100 calcium binding protein A14 antibody
Anti-S100A14 antibody (ab233189) at 2 µg/ml + Recombinant human S100A14
Predicted band size: 12 kDa
Anti-S100A14 antibody (ab233189) at 1 µg/ml + Pig stomach tissue lysate
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 12 kDa
Formalin-fixed, paraffin-embedded human skin cancer tissue stained for S100A14 using ab233189 at 20 µg/mL in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233189 has not yet been referenced specifically in any publications.